Structural analysis of α-carrageenan sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID
Structural analysis of α-carrageenan sulfated oligosaccharides by positive mode nano-ESI-FTICR-MS and MS/MS by SORI-CID
复制标题
DOI：
10.1016/j.jasms.2005.09.009
复制
发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
Lebrilla, CB
中科院分区：
文献类型：
--
作者：
Aguilan, JT;Dayrit, FM;Lebrilla, CB
采用正离子模式nano-ESI-FTICR-MS结合持续非共振辐射-碰撞诱导解离（SORI-CID）的MS/MS技术，成功地对卡拉胶中10个残基（分子量> 2 kDa）的硫酸寡糖进行了结构分析。观察到通过B-和Y-型片段化的糖苷键裂解反应，并能够对寡糖样品进行完全测序。使用SORI-CID可观察到不稳定的硫酸酯取代基的位置，从而能够确定硫酸化残基的序列。
Structural analysis of sulfated oligosaccharides from K-carrageenan of up to ten residues (MW > 2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.