Hydrogen peroxide accelerated the lignification process of bamboo shoots by activating the phenylpropanoid pathway and programmed cell death in postharvest storage
Hydrogen peroxide accelerated the lignification process of bamboo shoots by activating the phenylpropanoid pathway and programmed cell death in postharvest storage
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过氧化氢通过激活苯丙素途径和采后储存中的程序性细胞死亡来加速竹笋的木质化过程
DOI：
10.1016/j.postharvbio.2019.03.012
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发表时间：
2019-07-01
影响因子：
7
通讯作者：
Luo, Zisheng
中科院分区：
文献类型：
--
作者：
Li, Dong;Limwachiranon, Jarukitt;Luo, Zisheng
木质化是竹笋采后贮藏过程中衰老的典型特征。本研究将外源过氧化氢（H2O2）和二苯基碘化鎓（DPI）应用于采后竹笋，探讨H2O2在木质化过程中的调控作用。将竹笋浸泡在 10 mM H2O2 或 5 mM DPI 中 10 分钟，在 20 +/- 1 摄氏度下保存 12 天。 H2O2处理通过激活NADPH氧化酶加速内源H2O2的积累，而DPI处理抑制NADPH氧化酶活性，导致内源H2O2减少。贮藏12 d后，H2O2处理的芽的硬度和木质素含量分别比对照芽高4.79%和5.14%。然而，DPI 处理却显示出相反的效果，与对照组相比，硬度降低了 10.88%，木质素含量降低了 19.23%。高水平的内源H2O2激活PAL、C4H和4CL的活性，从而促进木质素生物合成。此外，H2O2 上调 DNase、RNase 和 caspase 3 样活性，导致程序性细胞死亡过程加速，最终导致木质化。透射电子显微镜进一步揭示了 H2O2 处理的芽中细胞壁增厚和细胞结构分裂。我们的研究结果表明，内源 H2O2 可能在竹笋的木质化过程中发挥着至关重要的作用。
Lignification is a typical characteristic of senescence of bamboo shoots during postharvest storage. In this study, exogenous hydrogen peroxide (H2O2) and diphenyliodonium iodide (DPI) were applied to postharvest bamboo shoots to explore the regulation of H2O2 in the lignification process. Bamboo shoots, soaked in 10 mM H2O2 or 5 mM DPI for 10 min, were stored at 20 +/- 1 degrees C for 12 d. H2O2 treatment accelerated the accumulation of endogenous H2O2 by activating NADPH oxidase, while DPI treatment inhibited NADPH oxidase activity, leading to the decrease of endogenous H2O2. After 12 d of storage, the firmness and lignin content in H2O2-treated shoots was 4.79% and 5.14% higher, respectively, than in the control shoots. However, DPI treatment showed the opposite effects, with 10.88% less firmness and 19.23% less lignin content observed as compared with the control group. High level of endogenous H2O2 activated the activities of PAL, C4H and 4CL, thus promoting lignin biosynthesis. Also, H2O2 upregulated DNase, RNase and caspase 3-like activities, leading to the acceleration of the programmed cell death process, which finally contributed to the resulting lignification. Transmission electron microscopy further revealed thickened cell walls and the decompartmentalization of cellular structure in H2O2-treated shoots. Our results indicated that endogenous H2O2 may play a vital role in the lignification process of bamboo shoots.