STIMULATION OF RADIAL EXPANSION IN ARABIDOPSIS ROOTS BY INHIBITORS OF ACTOMYOSIN AND VESICLE SECRETION BUT NOT BY VARIOUS INHIBITORS OF METABOLISM
STIMULATION OF RADIAL EXPANSION IN ARABIDOPSIS ROOTS BY INHIBITORS OF ACTOMYOSIN AND VESICLE SECRETION BUT NOT BY VARIOUS INHIBITORS OF METABOLISM
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DOI：
10.1007/bf00196673
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发表时间：
1995-10-01
期刊：
影响因子：
4.3
通讯作者：
BIVENS, NJ
中科院分区：
文献类型：
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作者：
BASKIN, TI;BIVENS, NJ
植物形态建成依赖于对生长各向异性的精确控制。为了了解生长各向异性的控制在多大程度上取决于细胞代谢，我们调查了生长根对一系列抑制剂的反应。拟南芥幼苗。（Heynh），7 - 8天龄，移植到含有抑制剂的平板上，并在随后的2天期间测量根的伸长和径向扩张。14种不同代谢过程的抑制剂抑制根伸长，但未能刺激径向扩张。这些抑制剂是硫酸铝、蚜虫菌素、（DNA合成），咖啡因（细胞板形成），顺铂（DNA合成），放线菌酮（蛋白质合成），3，4-脱氢-L-脯氨酸（脯氨酸羟基化），6-二甲基氨基嘌呤（蛋白激酶），二硝基苯酚（线粒体ATP合成），（UDP-葡萄糖半乳糖形成），洛伐他汀，原名美维诺林（类异戊二烯形成）、甲硫氨酸亚砜亚胺（谷氨酰胺合成酶）、甲氨蝶呤（叶酸代谢）、XRD-489（支链氨基酸的合成）和高或低钙处理。这些结果表明，各种类型的代谢中断，虽然抑制伸长，不降低高度的各向异性生长的根。然而，五种化学物质刺激径向扩张，即洗涤剂，毛地黄皂苷;两种抑制剂的囊泡分泌，莫能菌素和布雷菲德菌素A;和两种抑制剂的肌动球蛋白，细胞松弛素B和丁二酮单肟。由这些化合物（丁二酮单肟除外）诱导的最大径向膨胀大于乙烯引起的，和处理的根的形态不类似于乙烯处理的根。这些结果表明囊泡分泌和肌动球蛋白在控制各向异性膨胀中起作用。
Plant morphogenesis depends on accurate control over growth anisotropy. To learn to what extent the control of growth anisotropy depends on cellular metabolism, we surveyed the response of growing roots to a range of inhibitors. Seedlings of Arabidopsis thaliana L. (Heynh), 7-8 d old, were transplanted onto plates containing an inhibitor, and elongation and radial expansion of roots were measured over the subsequent 2-d period. Fourteen inhibitors of diverse metabolic processes inhibited root elongation but failed to stimulate radial expansion. These inhibitors were aluminum sulfate, aphidicolin (DNA synthesis), caffeine (cell-plate formation), cisplatin (DNA synthesis), cycloheximide (protein synthesis), 3,4-dehydro-L-proline (proline hydroxylation), 6-dimethyl-aminopurine (protein kinases), dinitrophenol (mitochondrial ATP synthesis), (UDP-glucose galactose formation), Lovastatin, formerly mevinolin (isoprenoid formation), methionine sulfoximine (glutamine synthetase), methotrexate (folate metabolism), XRD-489 (synthesis of branched-chain amino acids), and high or low calcium treatments. These results show that various types of metabolic disruption, although inhibitory to elongation, do not reduce the high degree of anisotropic growth of the root. However, five chemicals did stimulate radial expansion; namely, the detergent, digitonin; two inhibitors of vesicle secretion, monensin and brefeldin A; and two inhibitors of actomyosin, cytochalasin B and butanedione monoxime. The maximum radial expansion induced by these compounds (except butanedione monoxime) was greater than that caused by ethylene, and the morphology of treated roots did not resemble that of roots treated with ethylene. These results indicate that vesicle secretion and actomyosin play a role in controlling anisotropic expansion.