Effects of anesthetics on the function of orexin-1 receptors expressed in Xenopus oocytes
Effects of anesthetics on the function of orexin-1 receptors expressed in Xenopus oocytes
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DOI：
10.1159/000101713
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发表时间：
2007-01-01
期刊：
影响因子：
3.1
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Minami, Kouichiro;Uezono, Yasuhito;Ueta, Yoichi
下丘脑中含有神经肽食欲素的神经元与睡眠和觉醒的控制以及嗜睡症的病理学有关。在这项研究中，我们研究了挥发性麻醉剂，乙醇和静脉麻醉剂对食欲素-A诱导的钙激活的氯电流使用非洲爪蟾卵母细胞表达食欲素-1受体（OX 1 Rs）的影响。挥发性麻醉药异氟烷、恩氟烷和氟烷抑制1 μ mol/l orexin-A引起的Cl-电流。乙醇、静脉麻醉药戊巴比妥和氯胺酮也抑制食欲素- A的作用.所有测试的化合物的抑制作用显示是由OX 1 R功能的抑制引起的。这些结果至少可以部分解释它们的催眠作用。版权所有（c）2007 S. Karger AG，巴塞尔。
Neurons in the hypothalamus containing the neuropeptide orexin have been implicated in the control of sleep and wakefulness and in the pathology of narcolepsy. In this study, we investigated the effects of volatile anesthetics, ethanol and intravenous anesthetics on orexin-A-induced Ca2+-activated Cl- currents using Xenopus oocytes expressing orexin- 1 receptors ( OX1Rs). The volatile anesthetics isoflurane, enflurane and halothane inhibited Cl- currents elicited by 1-mu mol/l orexin-A. Ethanol and the intravenous anesthetics pentobarbital and ketamine also inhibited the action of orexin- A. The inhibitory effects of all of the compounds tested were shown to be caused by the inhibition of OX1R function. These results may, at least in part, explain their hypnotic effects. Copyright (c) 2007 S. Karger AG, Basel.