Lipid peroxidation and antioxidant status in experimental diabetes
Lipid peroxidation and antioxidant status in experimental diabetes
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DOI：
10.1016/s0009-8981(99)00046-7
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发表时间：
1999-06-15
影响因子：
5
通讯作者：
Mazur, A
中科院分区：
文献类型：
--
作者：
Feillet-Coudray, C;Rock, E;Mazur, A
氧化应激目前被认为是糖尿病的一种机制。本研究旨在用不同的互补方法评估链脲佐菌素诱导的糖尿病大鼠的氧化应激相关参数：体外氧化敏感性(肝脏匀浆中的脂质过氧化诱导，红细胞溶血)，血液抗氧化状态(两种方法的总抗氧化能力)，以及体内脂质过氧化的新标记物-血浆异前列腺素测定。我们发现，尽管肝脏维生素E含量增加，但诱导的肝脏硫代巴比妥酸反应物质在糖尿病4周后增加。糖尿病4周后红细胞溶血明显延迟。血浆抗氧化能力(AOC)在糖尿病4周后呈上升趋势，并与血浆维生素E水平呈正相关。总抗氧化活性(TAA)在1周后显著降低，并与血浆白蛋白水平显著相关。血浆异前列腺素(8-epi-prostaglandinF2α)浓度在糖尿病诱导后1周或4周无明显变化。饮食中维生素E的水平及其在体内分布的变化似乎在糖尿病期间氧化应激的发展及其后果中发挥了重要作用。(C)1999 Elsevier Science B.V.保留所有权利。
Oxidative stress is currently suggested as a mechanism underlying diabetes. The present study was designed to evaluate the oxidative stress related parameters in streptozotocin-induced diabetes in rats using different complementary approaches: susceptibility to in vitro oxidation (Lipid peroxidation induction in liver homogenate, red blood cells hemolysis), blood antioxidant status (total antioxidant capacity by two approaches), and plasma isoprostane measurement, a new marker of lipid peroxidation in vivo. We have shown that induced liver thiobarbituric acid reactive substances increased after 4 weeks of diabetes, in spite of increased liver vitamin E content. Red blood cells hemolysis was significantly delayed after 4 weeks of diabetes. Plasma antioxidant capacity (AOC) tended to increase after 4 weeks of diabetes and was correlated with plasma vitamin E levels. Total antioxidant activity (TAA) significantly decreased after 1 week and a significant correlation was observed with plasma albumin levels. Plasma isoprostane (8-epi-prostaglandinF2 alpha) concentrations were not modified significantly 1 week or 4 weeks after the induction of diabetes. Levels of vitamin E in the diet and changes in its distribution among the body seems to play an important role in the development of oxidative stress during diabetes and its consequences. (C) 1999 Elsevier Science B.V. All rights reserved.