The action of boiled pancreas extract on yeast nucleic acid
The action of boiled pancreas extract on yeast nucleic acid
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DOI：
10.1152/ajplegacy.1920.52.1.203
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发表时间：
1920-05-01
影响因子：
--
通讯作者：
Jones, W
中科院分区：
文献类型：
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作者：
Jones, W
最早的核酸研究表明，通过温和的酸水解，嘌呤与部分磷酸和部分碳水化合物一起释放;但是，需要剧烈的水解过程才能释放嘧啶与剩余的磷酸和碳水化合物。一大批天才。因此，不需要Y来制定核酸的假设结构。该物质必须由四种复合物组成，所有这些复合物都含有一个磷酸基团和一个碳水化合物基团，但每种复合物含有四个含氮基团中的不同基团。把“复合物”一词改为“核苷酸”一词，上述理论就基本上成为酵母核酸构成的现代核苷酸理论，但早期研究者没有触及有关酵母核酸构成的一个重要问题。核苷酸在什么点连接在一起形成酵母核酸，或者换句话说，酵母核酸中核苷酸的连接方式是什么？在没有任何实验证据的情况下，最终同意核苷酸连接是通过磷酸基团的，并且该假设保持不变，直到Jones、Germann和Read提供实验证据以表明这种核苷酸连接模式是不正确的。本文的主要目的是描述一个实验，它以一种非常简单而引人注目的方式证明了这一点。
The earliest investigations of nucleic acid had shown that by mild acid hydrolysis, the purines are set free with part of the phosphoric acid and part of the carbohydrate; but that violent hydrolytic processes are required to set free the pyrimidines with the remainder of the phosphoric acid and carbohydrate. A great amount of ingenuit. y was therefore not required to formulate a hypothetical structure for nucleic acid. The substance must be composed of four complexes, all of which contain a group of phosphoric acid and a group of carbohydrate but each complex contains a different one of the four nitrogenous groups. Change the word “complex” to the word “nucleotide” and the above becomes essentially the modern nucleotide theory of the constitution of yeast nucleic acid.But one important matter concerning the constitution of yeast nucleic acid was not touched upon by the early investigators. At what points are the nucleotides joined together to form yeast nucleic acid or, in other words, what is the mode of nucleotide linkage in yeast nucleic acid? Without any experimental evidence it was finally agreed that the nucleotide linkage is through the phosphoric acid groups and this assumption remained undisturbed until Jones, Germann and Read furnished the experimental evidence to show that this mode of nucleotide linkage is not correct. The principal object of the present paper is to describe an experiment which proves this in a very simple and striking way.