Muller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish
Muller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish
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DOI：
10.3791/56249
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发表时间：
2017-10-01
影响因子：
1.2
通讯作者：
Enzmann, Volker
中科院分区：
文献类型：
--
作者：
Conedera, Federica M.;Arendt, Petra;Enzmann, Volker
硬骨鱼和哺乳动物之间的一个有趣的区别是硬骨鱼视网膜在严重损伤后的视网膜神经发生和再生的终身潜力。研究斑马鱼的再生途径可能会为开发治疗哺乳动物视网膜退行性疾病的创新策略带来新的见解。在这里，我们专注于通过532 nm半导体激光诱导成年斑马鱼视网膜外层的局灶性病变。局部损伤允许研究直接在损伤区域的视网膜变性和再生期间发生的生物过程。使用非侵入性光学相干断层扫描（OCT），我们能够确定受损区域的位置，并监测随后的体内再生。实际上，OCT成像产生了斑马鱼视网膜的高分辨率横截面图像，提供了以前只能通过组织学分析获得的信息。为了证实来自实时OCT的数据，进行组织切片，并通过免疫组织化学研究诱导视网膜损伤后的再生反应。
A fascinating difference between teleost and mammals is the lifelong potential of the teleost retina for retinal neurogenesis and regeneration after severe damage. Investigating the regeneration pathways in zebrafish might bring new insights to develop innovative strategies for the treatment of retinal degenerative diseases in mammals. Herein, we focused on the induction of a focal lesion to the outer retina in adult zebrafish by means of a 532 nm diode laser. A localized injury allows investigating biological processes that take place during retinal degeneration and regeneration directly at the area of damage. Using non-invasive optical coherence tomography (OCT), we were able to define the location of the damaged area and monitor subsequent regeneration in vivo. Indeed, OCT imaging produces high-resolution, cross-sectional images of the zebrafish retina, providing information which was previously only available with histological analyses. In order to confirm the data from real-time OCT, histological sections were performed and regenerative response after the induction of the retinal injury was investigated by immunohistochemistry.