Experimental infection of ground squirrels (Spermophilius tridecemlineatus) with Monkeypox virus
Experimental infection of ground squirrels (Spermophilius tridecemlineatus) with Monkeypox virus
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DOI：
10.3201/eid1009.040310
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发表时间：
2004-09-01
影响因子：
11.8
通讯作者：
Xiao, SY
中科院分区：
文献类型：
--
作者：
Tesh, RB;Watts, DM;Xiao, SY
提出了一种新的小动物（啮齿动物）模型，用于研究严重正痘病毒感染的发病机制和治疗。用猴痘病毒（MPXV）经腹腔和鼻内感染13只黄鼠（Spermophilus tridecemlineatus）。所有动物均发生暴发性疾病，并在感染后6-9天死亡。在死亡前几天从血液和口咽培养病毒;在尸检时，所有测试的器官都含有相对较高的MPXV滴度。主要病理结果在肝脏，表现为小叶中心坏死、脂肪变性和肝细胞中的嗜碱性包涵体。还观察到脾坏死以及肺间质炎症。地松鼠MPXV的病理特征与猕猴MPXV和人类严重天花病毒感染的病理特征相似。
A proposed new small-animal (rodent) model for studying the pathogenesis and treatment of severe orthopoxvirus infections is described. Thirteen-lined ground squirrels (Spermophilus tridecemlineatus) were infected intraperitoneally and intranasally with monkeypox virus (MPXV). A fulminant illness developed in all animals, and they died 6-9 days after infection. Virus was cultured from the blood and oropharynx several days before death; at necropsy, all of the organs tested contained relatively high titers of MPXV. The major pathologic findings were in the liver, which showed centrilobular necrosis, steatosis, and basophilic inclusion bodies in hepatocytes. Splenic necrosis was also observed, as well as interstitial inflammation in the lungs. The pathologic features of MPXV in ground squirrels are similar to that described with MPXV in macaques and severe variola (smallpox) virus infection in humans.