Vibrational Raman optical activity of proteins, nucleic acids, and viruses
Vibrational Raman optical activity of proteins, nucleic acids, and viruses
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DOI：
10.1016/s1046-2023(02)00310-9
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发表时间：
2003-02-01
期刊：
影响因子：
4.8
通讯作者：
Barron, LD
中科院分区：
文献类型：
--
作者：
Blanch, EW;Hecht, L;Barron, LD
由于其对手性的敏感性，拉曼旋光性（罗阿）（其可以作为手性分子在右旋和左旋圆偏振入射光中的振动拉曼散射的微小差异来测量）是溶液中生物分子结构的有力探针。蛋白质罗阿光谱提供了多肽骨架的二级和三级结构、水合作用、侧链构象和变性状态下存在的结构元素的信息。核酸罗阿光谱产生关于糖环构象、碱基堆积排列以及围绕C-N糖苷键的糖环和碱基环的相互取向的信息。罗阿能够同时探测完整病毒的蛋白质和核酸组分的结构。本文简要介绍了罗阿光谱的理论和测量方法，并给出了蛋白质、核酸和病毒的罗阿光谱，说明了罗阿光谱在生物分子研究中的应用。(C)2003 Elsevier Science（美国）。All rights reserved.
Due to its sensitivity to chirality, Raman optical activity (ROA), which may be measured as a small difference in vibrational Raman scattering from chiral molecules in right- and left-circularly polarized incident light, is a powerful probe of biomolecular structure in solution. Protein ROA spectra provide information on the secondary and tertiary structures of the polypeptide backbone, hydration, side-chain conformation, and structural elements present in denatured states. Nucleic acid ROA spectra yield information on the sugar ring conformation, the base stacking arrangement, and the mutual orientation of the sugar and base rings around the C-N glycosidic linkage. ROA is able to simultaneously probe the structures of both the protein and the nucleic acid components of intact viruses. This article gives a brief account of the theory and measurement of ROA and presents the ROA spectra of a selection of proteins, nucleic acids, and viruses which illustrate the applications of ROA spectroscopy in biomolecular research. (C) 2003 Elsevier Science (USA). All rights reserved.