The integrative analysis of microRNA and mRNA expression in Apis mellifera following maze-based visual pattern learning
The integrative analysis of microRNA and mRNA expression in Apis mellifera following maze-based visual pattern learning
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基于迷宫的视觉模式学习后蜜蜂中 microRNA 和 mRNA 表达的综合分析
DOI：
10.1111/1744-7917.12065
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发表时间：
2014-10-01
期刊：
影响因子：
4
通讯作者：
Zeng, Zhi-Jiang
中科院分区：
文献类型：
--
作者：
Qin, Qiu-Hong;Wang, Zi-Long;Zeng, Zhi-Jiang
蜜蜂是一种感官能力强、行为类型多样的群居昆虫，被认为是研究学习记忆神经生物学基础的良好模式生物。在这项研究中，我们使用下一代小RNA测序和Solexa/llLumina数字基因表达标签分析(DGE)分析了迷宫视觉学习后microRNA(MiRNA)和Messenger RNA(MRNA)的变化。对于小RNA测序，迷宫组和对照组分别获得了13367770和13132655个干净标签。共检测到40个已知的差异表达miRNAs，迷宫组均较对照组表达上调。对于DGE，迷宫组和对照组分别检测到5 681 320和5 939 855个清洁标签。与对照组相比，迷宫组与对照组相比，共有388个差异表达基因，其中45个基因表达上调，343个基因表达下调。此外，定量逆转录聚合酶链式反应(qRT-PCR)证实了10个差异表达基因的表达水平，其中8个基因的表达趋势与DGE结果一致，尽管变化幅度较小。MiRNA和mRNA表达的综合分析表明，在40个已知差异表达的miRNAs和388个差异表达基因中，有60对miRNA/mRNAs在本研究中被鉴定为共表达。这些结果表明，miRNA和mRNA可能在蜜蜂的学习和记忆过程中发挥关键作用。我们的测序数据为基于迷宫的视觉学习提供了全面的miRNA和基因表达信息，这将有助于理解蜜蜂学习和记忆的分子机制。
The honeybee Opts mellifera) is a social insect with strong sensory capacity and diverse behavioral repertoire and is recognized as a good model organism for studying the neurobiological basis of learning and memory. In this study, we analyzed the changes in microRNA (miRNA) and messenger RNA (mRNA) following maze-based visual learning using next-generation small RNA sequencing and Solexa/lllumina Digital Gene Expression tag profiling (DGE). For small RNA sequencing, we obtained 13 367 770 and 13 132 655 clean tags from the maze and control groups, respectively. A total of 40 differentially expressed known miRNAs were detected between these two samples, and all of them were up-regulated in the maze group compared to the control group. For DGE, 5 681 320 and 5 939 855 clean tags were detected from the maze and control groups, respectively. There were a total of 388 differentially expressed genes between these two samples, with 45 genes up-regulated and 343 genes down-regulated in the maze group, compared to the control group. Additionally, the expression levels of 10 differentially expressed genes were confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and the expression trends of eight of them were consistent with the DGE result, although the degree of change was lower in amplitude. The integrative analysis of miRNA and mRNA expression showed that, among the 40 differentially expressed known miRNAs and 388 differentially expressed genes, 60 pairs of miRNA/mRNA were identified as co-expressed in our present study. These results suggest that both miRNA and mRNA may play a pivotal role in the process of learning and memory in honeybees. Our sequencing data provide comprehensive miRNA and gene expression information for maze-based visual learning, which will facilitate understanding of the molecular mechanisms of honeybee learning and memory.