Evidence for the association between IgG-antimitochondrial antibody and biochemical response to ursodeoxycholic acid treatment in primary biliary cholangitis
Evidence for the association between IgG-antimitochondrial antibody and biochemical response to ursodeoxycholic acid treatment in primary biliary cholangitis
复制标题
IgG 抗线粒体抗体与熊去氧胆酸治疗原发性胆汁性胆管炎生化反应之间相关性的证据
DOI：
10.1111/jgh.13534
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发表时间：
2017
影响因子：
4.1
通讯作者：
Hou Jinlin
中科院分区：
文献类型：
--
作者：
Tang Libo;Zhong Ruihua;He Xuanqiu;Wang Weibin;Liu Jinhong;Zhu Youfu;Li Yongyin;Hou Jinlin
背景与目的抗线粒体抗体（AMA）被认为是原发性胆汁性胆管炎（PBC）的血清学标志，但熊去氧胆酸（UDCA）治疗期间AMA的变化数据很少。在这里，我们评估了UDCA治疗对AMA滴度及其相关因素的影响productionsMethodsSerum伊加‐AMA，IgM‐AMA，IgG‐AMA，肿瘤坏死因子家族（BAFF）的B细胞活化因子，和循环浆母细胞的频率检测PBC患者，包括那些接受UDCA治疗24周，健康对照，慢性肝炎B患者，和自身免疫性肝炎患者。对对照组和PBC患者的连续肝切片进行免疫组化染色，检测肝内CD 38+、伊加+、IgM+和IgG+细胞。结果发现IgG‐AMA滴度的显著降低仅限于对UDCA治疗有生化反应的PBC患者（P= 0.005），并且在第24周定量循环浆母细胞（P= 0.025）和血清BAFF（P= 0.013）中也观察到类似的模式。PBC患者血清BAFF水平与IgG‐AMA滴度、循环浆母细胞数呈正相关（r= 0.464，P = 0.034; r = 0.700，P < 0.001）。此外，原位染色显示PBC liver.ConclusionsDecreased滴度降低血清IgG-AMA的门静脉束内的CD 38+和IgG+细胞的显著积累与生化反应UDCA治疗，这意味着潜在的治疗反应评估和有益的影响，UDCA对PBC患者的体液免疫标志。
Background and AimAntimitochondrial antibody (AMA) is considered the serological hallmark of primary biliary cholangitis (PBC), while data regarding the profile of AMA during ursodeoxycholic acid (UDCA) treatment are scarce. Here, we assessed the influence of UDCA treatment on titers of AMA and factors relevant to its production.MethodsSerum IgA‐AMA, IgM‐AMA, IgG‐AMA, B cell‐activating factor of the tumor necrosis factor family (BAFF), and the frequency of circulating plasmablasts were detected in PBC patients, including those who received UDCA therapy for 24 weeks, healthy controls, chronic hepatitis B patients, and autoimmune hepatitis patients. Consecutive liver sections from controls and PBC patients were stained by immunohistochemistry for detection of intrahepatic CD38+, IgA+, IgM+, and IgG+cells.ResultsSignificant decrease in titers of IgG‐AMA was found only confined to PBC patients with biochemical response to UDCA treatment (P= 0.005), and similar pattern was also observed at week 24 in quantifying circulating plasmablasts (P= 0.025) and serum BAFF (P= 0.013). Notably, positive correlation between serum BAFF levels and titers of IgG‐AMA, and the frequency of circulating plasmablasts were observed in PBC patients (r= 0.464,P= 0.034 andr= 0.700,P< 0.001, respectively). Additionally,in situstaining revealed significant accumulation of CD38+and IgG+cells within the portal tracts of PBC liver.ConclusionsDecreased titers of serum IgG‐AMA are associated with biochemical response to UDCA treatment, implicating the potentiality of this hallmark in therapeutic response evaluation and the beneficial effect of UDCA on humoral immunity in PBC patients.