Suppression of diabetes in non-obese diabetic (NOD) mice by oral administration of water-soluble and alkali-soluble polysaccharide conjugates prepared from green tea
Suppression of diabetes in non-obese diabetic (NOD) mice by oral administration of water-soluble and alkali-soluble polysaccharide conjugates prepared from green tea
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口服绿茶制备的水溶性和碱溶性多糖缀合物抑制非肥胖糖尿病（NOD）小鼠的糖尿病
DOI：
10.1016/j.carbpol.2010.04.017
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发表时间：
2010-08-02
影响因子：
11.2
通讯作者：
Wan, Haitong
中科院分区：
文献类型：
--
作者：
Chen, Xiaoqiang;Lin, Zhi;Wan, Haitong
分别用热水和碱水从绿色茶叶中提取水溶性和碱溶性茶多糖结合物（TPC-W和TPC-A）。分析了TPC-W和TPC-A的理化性质。采用非肥胖糖尿病（NOD）小鼠评价TPC-W和TPC-A的抗糖尿病生物活性。每天口服150 mg kg（-1）TPC-W可显著降低NOD小鼠的血糖水平。150 mg·kg ~（-1）TPC-W可使NOD小鼠抗谷氨酸脱羧酶（GAD）抗体水平下降27%（P < 0.05）。试验结束时，接受TPC-W或TPC-A治疗的NOD组小鼠中，只有2/10的小鼠出现糖尿病症状，而模型对照组小鼠中，有7/10的小鼠出现糖尿病。脏器指数测定结果表明，TPC-W和TPC-A对胸腺萎缩均有一定的保护作用。总之，我们的研究表明TPC-W和TPC-A都可以抑制NOD小鼠的自发性糖尿病。(C)2010爱思唯尔有限公司版权所有。
Water-soluble tea polysaccharide conjugates (TPC-W) and alkali-soluble tea polysaccharide conjugates (TPC-A) were extracted from green tea by hot and alkali water respectively. Physicochemical properties of TPC-W and TPC-A were analyzed. Non-obese diabetic (NOD) mice were used to evaluate antidiabetic bioactivities of TPC-W and TPC-A. The daily oral administration of 150 mg kg(-1) TPC-W can significantly decrease the level of blood glucose in NOD mice. The anti-glutamic acid decarboxylase (GAD) antibody level in NOD mice treated with 150 mg kg-1 TPC-W decreased 27% (P < 0.05). To the end of trial, only 2 out of 10 mice in NOD groups treated with TPC-W or TPC-A exhibited diabetic symptoms compared with model control group, in which 7 of 10 mice developed diabetes. The result of organ index showed that both TPC-W and TPC-A can protect thymus from shriveling to some extent. In sum, our studies demonstrated that both TPC-W and TPC-A can suppress spontaneous diabetes mellitus in NOD mice. (C) 2010 Elsevier Ltd. All rights reserved.