Quantification of HTLV-1 proviral copy number in peripheral blood of symptomless carriers from the French West Indies.
Quantification of HTLV-1 proviral copy number in peripheral blood of symptomless carriers from the French West Indies.
复制标题
来自法属西印度群岛的无症状携带者外周血中 HTLV-1 前病毒拷贝数的定量。
DOI：
--
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
J. Lefrère
中科院分区：
文献类型：
--
作者：
E. Wattel;M. Mariotti;F. Agis;E. Gordien;Levchenko Ff;L. Prin;Phillipe Rouger;I. Chen;S. Wain;J. Lefrère
人类T细胞白血病1型（HTLV-1）疾病的发展与外周血单核细胞（PBMC）中前病毒拷贝数（VCN）的增加有关。研究了20名无症状抗HTLV-1阳性献血者，以及4名有症状的个体，均来自法属西印度群岛。通过定量PCR测定PBMCs中的VCN。无症状HTLV-1携带者的VCN值（范围小于100至约9，500/微克DNA）几乎总是小于有症状携带者的值（范围约为5，500至约29，000/微克DNA）。因此，HTLV-1感染的PBMC在无症状个体和有症状个体中的比例范围分别为小于1/1，500至约1/16和约1/27至约1/5。未发现VCN与年龄或性别之间的相关性，表明年龄和性别以外的因素对VCN数量的影响的重要性。
The development of human T-cell leukemia type 1 (HTLV-1) diseases are related to an increase in the proviral copy number (VCN) in peripheral blood mononuclear cells (PBMCs). Twenty symptomless anti-HTLV-1-positive blood donors, as well as four symptomatic individuals, all from the French West Indies, were studied. The VCN in PBMCs was determined by quantitative PCR. The VCN values for asymptomatic HTLV-1 carriers (range of less than 100 to approximately 9,500/micrograms of DNA) was nearly always less than the values for symptomatic carriers (range of approximately 5,500 to approximately 29,000/micrograms of DNA). Consequently, the proportion of HTLV-1-infected PBMCs in symptomless and in symptomatic individuals ranged from less than 1/1,500 to approximately 1/16 and approximately 1/27 to approximately 1/5, respectively. No correlation could be found between VCN and age or sex, suggesting the importance of factors other than age and sex as influences on the VCN number.