Variations in local elastic modulus along the length of the aorta as observed by use of a scanning haptic microscope (SHM)
Variations in local elastic modulus along the length of the aorta as observed by use of a scanning haptic microscope (SHM)
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DOI：
10.1007/s10047-011-0596-2
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发表时间：
2011-12-01
影响因子：
1.3
通讯作者：
Nakayama, Yasuhide
中科院分区：
文献类型：
--
作者：
Moriwaki, Takeshi;Oie, Tomonori;Nakayama, Yasuhide
用扫描触觉显微镜(SHM)评价犬主动脉全长的微观弹性结构的变化。从腹主动脉弓至腹主动脉的全主动脉分为6个大致相等的节段。将主动脉包埋于琼脂中后，切成水平的周段，得到含有平坦表面(厚度约1 mm)的环状主动脉标本的盘状琼脂部分。使用直径为5微米、空间分辨率为2微米的探头，以0.3 S/点的速度，沿墙体厚度方向同时测量了试件在空载条件下的弹性模量和表面形貌。管壁的弹性模数在管腔表面一侧最高，向外膜侧逐渐减小。这一趋势与弹性蛋白纤维含量的变化相似。在评估每段中膜中段的中段时，发现弹性纤维密度最高的主动脉胸段的弹性模数最高(40.8+/-3.5kPa)。在空载条件下，主动脉弹性蛋白密度高的部分具有较高的弹性模数。
Variations in microscopic elastic structures along the entire length of canine aorta were evaluated by use of a scanning haptic microscope (SHM). The total aorta from the aortic arch to the abdominal aorta was divided into 6 approximately equal segments. After embedding the aorta in agar, it was cut into horizontal circumferential segments to obtain disk-like agar portions containing ring-like samples of aorta with flat surfaces (thickness, approximately 1 mm). The elastic modulus and topography of the samples under no-load conditions were simultaneously measured along the entire thickness of the wall by SHM by using a probe with a diameter of 5 mu m and a spatial resolution of 2 mu m at a rate of 0.3 s/point. The elastic modulus of the wall was the highest on the side of the luminal surface and decreased gradually toward the adventitial side. This tendency was similar to that of the change in the elastin fiber content. During the evaluation of the mid-portion of each tunica media segment, the highest elastic modulus (40.8 +/- A 3.5 kPa) was identified at the thoracic section of the aorta that had the highest density of elastic fibers. Under no-load conditions, portions of the aorta with high elastin density have a high elastic modulus.