Antibody to neural cell adhesion molecule can disrupt the migration of luteinizing hormone-releasing hormone neurons into the mouse brain.
Antibody to neural cell adhesion molecule can disrupt the migration of luteinizing hormone-releasing hormone neurons into the mouse brain.
复制标题
神经细胞粘附分子的抗体可以破坏黄体生成素释放激素神经元迁移到小鼠大脑中。
DOI：
10.1002/cne.903420203
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Pfaff,DW
中科院分区：
文献类型：
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作者：
Schwanzel-Fukuda,M;Reinhard,GR;Abraham,S;Crossin,KL;Edelman,GM;Pfaff,DW
合成和释放促黄体激素释放激素（LHRH）的神经元被假设起源于内侧嗅窝的上皮，并沿着由终末和犁鼻神经的神经细胞粘附分子（NCAM）免疫反应性分支组成的支架迁移到大脑中。通过免疫细胞化学和放射自显影程序研究发现，这些LHRH神经元起源于小鼠胚胎发育的非常短的时期内，特别是第10天，并且沿着迁移路线沿着显著有序的时空过程进入大脑。本实验的目的是确定在发育的特定时间，会阻止LHRH神经元迁移到大脑我们发现，在胚胎发育的第10天，将1 μl NCAM抗血清注射到嗅窝区域，显著减少了内侧嗅窝上皮中观察到的LHRH免疫反应阳性神经元的数量，同时在迁移路线上观察到基板外的LHRH免疫反应阳性细胞的数量也显著减少。这些结果证实了我们最初的假设，即LHRH神经元迁移从上皮细胞的嗅窝到大脑，并表明，NCAM在这种现象中起着因果关系的作用。© 1994 Wiley利斯公司
The neurons which synthesize and release luteinizing hormone‐releasing hormone (LHRH), are hypothesized to originate in the epithelium of the medial olfactory pit and to migrate into the brain along a scaffolding made up of neural cell adhesion molecule (NCAM)‐immunoreactive branches of the terminal and vomeronasal nerves. These LHRH neurons, studied by immunocytochemical and autoradiographic procedures, were found to originate within a very short period of embryogenesis, specifically day 10, in mice, and to follow a remarkably ordered spatiotemporal course along the migration route into the brain.The purpose of the present experiments was to determine whether perturbation of the NCAM‐immunoreactive migration route, at a particular time in development, would arrest the migration of LHRH neurons into the brain. We found that a 1 μl injection of antiserum to NCAM into the area of the olfactory pit, on day 10 of embryogenesis, significantly reduced the number of LHRH‐immunoreactive neurons seen in the epithelium of the medial olfactory pit, with a concomitant significant reduction in the number of LHRH‐immunoreactive cells seen outside of the placode, on the migration route. These results confirm our initial hypothesis that LHRH neurons migrate from the epithelium of the olfactory pit to the brain and indicate that NCAM plays a causal role in this phenomenon. © 1994 Wiley‐Liss, Inc.