Transovarial transmission of DENV in Aedes aegypti in the Amazon basin: a local model of xenomonitoring.
Transovarial transmission of DENV in Aedes aegypti in the Amazon basin: a local model of xenomonitoring.
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DOI：
10.1186/s13071-017-2194-5
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发表时间：
2017-05-19
影响因子：
3.2
通讯作者：
Pimenta PFP
中科院分区：
文献类型：
--
作者：
da Costa CF;Dos Passos RA;Lima JBP;Roque RA;de Souza Sampaio V;Campolina TB;Secundino NFC;Pimenta PFP
登革病毒在伊蚊中的跨卵传播。蚊子被认为是病毒在自然界中维持的重要机制，并可能与疾病的爆发和流行有关。然而，很少有研究涉及跨卵传播和病毒载体监测作为监测工具和控制策略。本研究评估了登革热病毒在埃及伊蚊种群中的跨卵传播，作为亚马孙州城市的异种监测策略。 收集伊蚊卵（13.164），三龄和四龄幼虫的存活率为30%。在所有城市都检测到登革病毒的跨卵传播。各市的经卵巢感染率（TOR）为DENV阳性样本的46%。该州的最低感染率为17.7，在各市测试的每1 000只幼虫中，感染率从11.4到24.1不等。确定了四种DENV血清型，其中DENV I和IV存在于所有调查的城市。在此期间，报告的登革热病例数量各不相同。我们的研究结果表明，跨卵巢传播可能是一个重要的机制，在亚马逊自治市的维护和传播的疾病。使用qRT-PCR，可以鉴定幼虫样品中的四种DENV血清型。本研究中使用的方法证明适合作为未成熟蚊子中的DENV异种监测模型，有助于开发用于病毒循环的早期检测系统和用于该疾病爆发和流行的预测模型。 CAAE34025414200005015。
Transovarial transmission of dengue virus in Aedes spp. mosquitoes is considered an important mechanism for the maintenance of the virus in nature and may be implicated in the occurrence of outbreaks and epidemics of the disease. However, there are few studies involving transovarial transmission and viral vector monitoring as a surveillance tool and control strategy. The present study evaluated transovarial transmission of dengue virus in Aedes aegypti populations as a xenomonitoring strategy in municipalities of the Amazonas state. Aedes sp. eggs (13.164) were collected, with 30% viability of third- and fourth-instar larvae. Transovarial transmission of DENV was detected in all municipalities. The transovarial infection rate (TOR) in the municipalities was 46% of the DENV positive samples. The minimum infection rate (MIR) was 17.7 in the state, varying from 11.4 to 24.1 per 1,000 larvae tested in the respective municipalities. Four DENV serotypes were identified, with DENV I and IV being present in all municipalities investigated. The number of reported dengue fever cases varied during this period. Our results suggest that transovarial transmission may be an important mechanism for the maintenance and spreading of the disease in Amazonas municipalities. Using qRT-PCR, it was possible to identify the four DENV serotypes in larval samples. The methodology used in the present study proved suitable as a DENV xenomonitoring model in immature mosquitoes, contributing to the development of systems for early detection of viral circulation and predictive models for the occurrence of outbreaks and epidemics of this disease. CAAE34025414200005015.