MiR-320a is Downregulated in Patients with Myasthenia Gravis and Modulates Inflammatory Cytokines Production by Targeting Mitogen-activated Protein Kinase 1
MiR-320a is Downregulated in Patients with Myasthenia Gravis and Modulates Inflammatory Cytokines Production by Targeting Mitogen-activated Protein Kinase 1
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DOI：
10.1007/s10875-012-9834-5
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发表时间：
2013-04-01
影响因子：
9.1
通讯作者：
Liu, Jianwen
中科院分区：
文献类型：
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作者：
Cheng, Zhuoan;Qiu, Shaobo;Liu, Jianwen
重症肌无力（Myasthenia gravis，MG）是T细胞依赖性抗体介导的自身免疫性疾病，microRNA是人类自身免疫性疾病发病机制的重要调节因子。在此，我们首次研究了MG中的miRNAs表达谱，发现与正常健康人相比，MG患者中的miR-320 a显著下调。同时，MG患者的促炎细胞因子过表达。此外，我们确定MAPK 1是miR-320 a的直接靶点。下调miR-320 a可通过促进考克斯-2的表达而诱导促炎细胞因子的过度表达。这一过程受到ERK/NF-κ B通路的调节。综上所述，我们的研究结果表明，miR-320 a可能在调节炎症细胞因子的产生中发挥作用。
Myasthenia gravis (MG) are T-cell dependent antibody-mediated autoimmune disorders, microRNAs are important regulators of human autoimmune disease pathogenesis. Here, we investigated the miRNAs expression profiles in MG for the first time and found that miR-320a was significantly downregulated in MG patients compared to normal healthy people. Meanwhile, pro-inflammatory cytokins in MG patients were overexpressed. Furthermore, we identified MAPK1 as a direct target of miR-320a. Downregulation of miR-320a induced the overexpression of pro-inflammatory cytokins through promoting COX-2 expression. This process was modulated by ERK/NF-kappa B pathways. Taken together, our findings suggested that miR-320a could play a role in modulation of inflammatory cytokins production.