Chemiluminescence immunoassay using magnetic nanoparticles with targeted inhibition for the determination of ochratoxin A
Chemiluminescence immunoassay using magnetic nanoparticles with targeted inhibition for the determination of ochratoxin A
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DOI：
10.1016/j.talanta.2015.03.044
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发表时间：
2015-08-01
期刊：
影响因子：
6.1
通讯作者：
Lim, H. B.
中科院分区：
文献类型：
--
作者：
Kim, Sumin;Lim, H. B.
在这项工作中，开发了一种具有靶向抑制作用的化学发光（CL）免疫测定法，用于测定食品中的毒素。对于样品处理，合成了胺功能化磁性纳米粒子（MNP）来提取目标分子，并使用标记在抗体上的辣根过氧化物酶（HRP）作为CL反应的标记。特别是，使用醛进行胺靶向抑制，即用烷基特异性封端胺，被开发用于非特异性提取平台，以降低背景并提高信号背景比。为了进行演示，使用实验室构建的滴式化学发光 (DCL) 系统和鲁米诺-H2O2 试剂测定了大米中的赭曲霉毒素 A (OTA)。获得的检出限为139 pg mL(-1)，比ELISA提高了约7.3倍。与ELISA相比，该方法的回收率在87-99%范围内。 (C) 2015 Elsevier B.V. 保留所有权利。
In this work, a chemiluminescence (CL) immunoassay with targeted inhibition was developed for the determination of toxins in food products. For sample treatment, amine-functionalized magnetic nanoparticles (MNPs) were synthesized to extract target molecules, and horseradish peroxidase (HRP) tagged on an antibody was used as a label for CL reaction. In particular, amine-targeted inhibition using aldehyde, i.e., specifically capping the amine with an alkyl group, was developed for a non-specific extraction platform to lower background and improve signal-to-background ratio. For demonstration, ochratoxin A (OTA) was determined in rice using a lab-built drop-type chemiluminescence (DCL) system with luminol-H2O2 reagent. The obtained limit of detection was 139 pg mL(-1), which was about 7.3 times better than that of ELISA. Recovery of the method in the range of 87-99% was observed, which was compared with ELISA. (C) 2015 Elsevier B.V. All rights reserved.