Prevalence and antibiotic susceptibility patterns of Shigella and Salmonella among children aged below five years with Diarrhoea attending Nigist Eleni Mohammed memorial hospital, South Ethiopia
Prevalence and antibiotic susceptibility patterns of Shigella and Salmonella among children aged below five years with Diarrhoea attending Nigist Eleni Mohammed memorial hospital, South Ethiopia
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DOI：
10.1186/s12887-018-1221-9
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发表时间：
2018-07-25
期刊：
影响因子：
2.4
通讯作者：
Godebo, Girma
中科院分区：
文献类型：
--
作者：
Abebe, Wondwossen;Earsido, Alemu;Godebo, Girma
背景：腹泻病是5岁以下儿童死亡的第二大原因。尽管这些疾病都是可预防和可治疗的，但全球仍有近17亿例儿童腹泻病例，每年造成约525 000名儿童死亡。志贺氏菌和沙门氏菌是腹泻相关死亡的主要病原体。本研究的目的是确定志贺氏菌和沙门氏菌的患病率和抗生素敏感性模式分离自5岁以下的腹泻儿童参加Nigist Eleni Mohammed纪念医院，Hossana，南Ethiopia.Methods：一项横断面研究，从2017年6月2日至9月24日进行。采用方便抽样技术连续入选204名5岁以下腹泻儿童。按照标准细菌学方法处理粪便标本，并使用纸片扩散法测定分离株的抗生素敏感性模式。结果：204例5岁以下儿童腹泻患儿中，细菌培养阳性19例（9.3%，95%CI5.7 -13.7%），其中志贺菌17例（8.3%），沙门菌2例（1%）。志贺菌和沙门菌对诺氟沙星、萘啶酸和卡那霉素的敏感率均为100%。志贺菌对氨苄西林、庆大霉素和复方新诺明的耐药率分别为100%、76.5%和64.7%，沙门菌对氨苄西林和庆大霉素的耐药率均为100%。在测试的抗生素中，诺氟沙星，萘啶酸和卡那霉素被认为是最有效的两个菌株。这两个物种都对常用的抗生素产生了抗药性。因此，基于培养的细菌种类鉴定和抗菌药物敏感性测试服务，强烈建议，以避免在研究领域的经验性治疗。
Background: Diarrhoeal disease is the second leading cause of death among children aged below 5 years. Even though, both preventable and treatable diseases, globally there are nearly 1.7 billion cases of childhood diarrhoeal disease and responsible for killing around 525,000 children every year. Shigella and Salmonella species were the leading cause of etiologic agents for diarrhoea associated deaths. The aim of this study was to determine the prevalence and antibiotic susceptibility patterns of Shigella and Salmonella isolated from children aged below 5 years with diarrhoea attending Nigist Eleni Mohammed Memorial Hospital, Hossana, South Ethiopia.Methods: A cross sectional study was conducted from June 02 to September 24, 2017. Two hundred four children aged below 5 years with diarrhoea were enrolled consecutively using convenience sampling technique. Stool specimens were processed in accordance with the standard bacteriological methods and antibiotic susceptibility pattern of the isolates was determined using disc diffusion method. Data were analyzed using SPSS version 20.Results: Out of the 204 children aged below 5 years with diarrhoeal disease 19/204 (9.3%, [95% CI, 5.7-13.7%]) of them were positive for bacterial growth, of which 17/204(8.3%) were Shigella species and 2/204(1%) were Salmonella species. Both Shigella and Salmonella isolates were 100% susceptible to norfloxacin, nalidixic acid and kanamycin. However, isolates of Shigella showed 100, 76.5 and 64.7% resistance to ampicillin, gentamicin and cotrimoxazole respectively while Salmonella species were highly resistant to ampicillin and gentamicin (100% each).Conclusions: Salmonella and Shigella species is prevalent in the current study area. Among the tested antibiotics, norfloxacin, nalidixic acid and kanamycin were found to be most effective for both isolates. Both species are developing resistance to the commonly prescribed antibiotic. Therefore, culture based bacterial species identification and antimicrobial susceptibility testing services are strongly recommended to avoid empirical treatment in the study area.