Induction of transforming growth factor-beta 1 on dentine pulp cells in different culture patterns
Induction of transforming growth factor-beta 1 on dentine pulp cells in different culture patterns
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DOI：
10.1016/j.cellbi.2005.12.001
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发表时间：
2006-04-01
影响因子：
3.9
通讯作者：
Jin, Y
中科院分区：
文献类型：
--
作者：
Nie, X;Tian, WD;Jin, Y
最近的研究表明，TGF-β 1参与牙髓组织修复。此外，牙髓细胞具有分化为成牙本质细胞样细胞的潜能，并在此过程中产生修复性牙本质。然而，由于牙髓/牙本质微环境的复杂性，TGF-β 1和牙髓细胞之间的分子机制和潜在的相互作用尚不清楚。本研究采用细胞培养、组织培养和三维培养的方法，研究了TGF-β 1对牙髓细胞的诱导作用。这些结果表明，TGF-β 1显着增加细胞的增殖和活性的ALP 4。在TGF-β 1存在下培养的牙髓细胞形成矿化结节。在器官培养中，TGF-β 1处理的牙髓细胞分化为成牙本质细胞样细胞并形成牙髓-牙本质复合体，并且TGF-β 1显著诱导牙本质相关蛋白DSPP、TGF-β 1的合成。牙髓细胞具有成牙本质细胞的一些特征，如平行排列的柱状细胞和单侧的细胞突起。这些数据表明，TGF-β 1可以使牙髓细胞分化为成牙本质细胞样细胞，并形成牙髓牙本质复合体。此外，这些结果表明，TGF-β 1是牙齿发育和牙髓修复过程中成牙本质细胞分化的重要调节因子。(c)2005年国际细胞生物学报告。由爱思唯尔有限公司出版。保留所有权利。
Recent studies have documented that TGF-beta 1 takes part in dental pulp tissue repair. Moreover, dental pulp cells have the potential to differentiate into odontoblast-like cells and produce reparative dentine in this process. However, the molecular mechanisms and potential interactions between TGF-beta l and dental pulp cells are not clear due to the complexity of the pulp/dentine microenvironment. In this study, we investigated the induction of TGF-beta 1 on the dental pulp cells in cell culture, tissue culture and three-dimensional culture patterns. These results demonstrated that TGF-beta 1 significantly increased the proliferation of cells and activity of ALPase. Dental pulp cells cultured in the presence of TGF-beta 1 formed mineralization nodules. In the organ culture, dental pulp cells treated with TGF-beta 1 differentiated into odontoblast-like cells and formed a pulp-dentinal complex: and TGF-beta 1 significantly induced synthesis of dentine relative proteins DSPP, DMP-1. The dental pulp cells share some characteristics of the odontoblast, Such as a parallel arrangement with columnar form and a unilateral cell process. Together, these data indicate that TGF-beta l can make dental pulp cells differentiated into odontoblast-like cells and form the pulp-dentinal complex. Moreover, these results suggest that TGF-beta l is an important regulatory factor in odontoblast differentiation during tooth development and pulp repair. (c) 2005 International rederation for Cell Biology. Published by Elsevier Ltd. All rights reserved.