Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation.
Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation.
复制标题
DOI：
10.1155/2016/9672082
复制
发表时间：
2016
影响因子：
--
通讯作者：
Bonini S
中科院分区：
文献类型：
--
作者：
Micera A;Di Zazzo A;Esposito G;Sgrulletta R;Calder VL;Bonini S
Objective.春季角结膜炎是一种与特应性角膜炎相关的慢性复发性结膜炎。几种炎症和组织重塑因子有助于VKC疾病。目的是提供一种基于芯片的蛋白质分析，从患者的眼泪患有静止或活动VKC。方法.该研究队列包括16例连续VKC患者和10例对照。参与者接受眼表和泪液取样的临床评估。评估总蛋白定量、总蛋白草图和蛋白阵列（六十种蛋白候选物）。结果在VKC阵列中观察到总体增加的荧光强度表达。尤其是IL 1 β、IL 15、IL 21、Eotaxin 2、TACE、MIP 1 α、MIP 3 α、NCAM 1、ICAM 2、βNGF、NT 4、BDNF、βFGF、SCF、MMP 1和MMP 2在静止期VKC中升高。在这些候选者中，只有IL 1 β、IL 15、IL 21、βNGF、SCF、MMP 2、Eotaxin 2、TACE、MIP 1 α、MIP 3 α、NCAM 1和ICAM 2在活动期和静止期VKC中均升高。最后，NT 4、βFGF和MMP 1在活动性VKC中高度增加。结论一个独特的“蛋白质撕裂纹”的特点VKC活动，证实了一些以前报道的因素，并突出了一些新的候选人共同的静止和活动状态。在静止VKC中表达的那些候选者可以被认为是VKC再激活和/或季节外加重的预测指标。
Objective. Vernal keratoconjunctivitis (VKC) is a chronic recurrent bilateral inflammation of the conjunctiva associated with atopy. Several inflammatory and tissue remodeling factors contribute to VKC disease. The aim is to provide a chip-based protein analysis in tears from patients suffering from quiescent or active VKC. Methods. This study cohort included 16 consecutive patients with VKC and 10 controls. Participants were subjected to clinical assessment of ocular surface and tear sampling. Total protein quantification, total protein sketch, and protein array (sixty protein candidates) were evaluated. Results. An overall increased Fluorescent Intensity expression was observed in VKC arrays. Particularly, IL1β, IL15, IL21, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, ICAM2, βNGF, NT4, BDNF, βFGF, SCF, MMP1, and MMP2 were increased in quiescent VKC. Of those candidates, only IL1β, IL15, IL21, βNGF, SCF, MMP2, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, and ICAM2 were increased in both active and quiescent VKC. Finally, NT4, βFGF, and MMP1 were highly increased in active VKC. Conclusion. A distinct “protein tear-print” characterizes VKC activity, confirming some previously reported factors and highlighting some new candidates common to quiescent and active states. Those candidates expressed in quiescent VKC might be considered as predictive indicators of VKC reactivation and/or exacerbation out-of-season.