CD4-regulatory cells in COPD patients
CD4-regulatory cells in COPD patients
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DOI：
10.1378/chest.07-0083
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发表时间：
2007-07-01
期刊：
影响因子：
9.6
通讯作者：
Singh, Dave
中科院分区：
文献类型：
--
作者：
Smyth, Lucy J. C.;Starkey, Cerys;Singh, Dave
背景：COPD患者气道CD 8和B淋巴细胞数量增加，提示存在自身免疫过程。目的：比较慢性阻塞性肺疾病（COPD）患者、正常肺功能的吸烟者和健康非吸烟者（HNS）的支气管肺泡灌洗液（BAL）和外周血单个核细胞（PBMC）中CD4调节性T细胞的数量。流式细胞术进行调节表型markets.Results：COPD患者BALCD8数量增加相比，吸烟者和HNS。两组之间的CD4数量相似。与HNS（中位数0%）相比，吸烟者（中位数28.8%）和COPD患者（中位数23.1%）的BAL CD4CD25（亮）表达增加。在BAL CD 4 CD 25（亮）细胞中证实了FoxP3表达增加。BALCD4CD25细胞表达较少的CD27相比，PBMCs，表明较弱的功能性regulatory ability.Clinical implications：慢性香烟烟雾暴露上调气道CD4调节细胞的数量。它们的功能可能是控制肺部炎症。
Background: The numbers of airway CD8 and B lymphocytes are increased in COPD patients, suggesting an autoimmune process. CD4-regulatory T cells control autoimmunity but have not been studied in patients with COPD.Objective: To compare T-regulatory cell numbers in the BAL from COPD patients, smokers with normal lung function, and healthy nonsmokers (HNS).Methods: BAL and peripheral blood mononuclear cell (PBMC) samples were obtained from 26 COPD patients, 19 smokers, and 8 HNS. Flow cytometry was performed for regulatory phenotypic markers.Results: COPD patients had increased BAL CD8 numbers compared to smokers and HNS. CD4 numbers were similar between groups. There was increased BAL CD4CD25(bright) expression in smokers (median 28.8%) and COPD patients (median 23.1%) compared to HNS (median 0%). Increased FoxP3 expression was confirmed in BAL CD4CD25(bright) cells. BAL CD4CD25 cells expressed less CD27 compared to PBMCs, suggesting weaker functional regulatory ability.Clinical implications: Chronic cigarette smoke exposure up-regulates airway CD4 regulatory cell numbers. Their function may be to control pulmonary inflammation.