Evolutionary Development and Expression Pattern of the Myeloid Lectin-Like Receptor Gene Family Encoded Within the NK Gene Complex
Evolutionary Development and Expression Pattern of the Myeloid Lectin-Like Receptor Gene Family Encoded Within the NK Gene Complex
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DOI：
10.1111/j.1365-3083.2010.02433.x
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发表时间：
2010-10-01
影响因子：
3.7
通讯作者：
Hofer, E.
中科院分区：
文献类型：
--
作者：
Sattler, S.;Ghadially, H.;Hofer, E.
自然杀伤（NK）基因复合物内的髓样簇包括在免疫系统中具有不同和高度重要功能的几种C型凝集素样受体基因，如LOX-1和DECTIN-1。基于已通过全基因组测序获得的序列，我们对人类、黑猩猩、小鼠和大鼠NK基因复合体进行了比较，以更好地表征该基因家族和该区域的其他基因在复合体内的系统发育关系和进化。我们发现，灵长类动物集群内的基因排列不同于啮齿类复合体中相应基因的顺序和方向，这可以用进化重复和反转事件来解释。对单个基因的分析揭示了支持编码蛋白质的首要重要性的高序列保守性。最近描述的CLEC 12 B和CLEC 9A基因的表达分析显示，不仅在单核细胞和树突细胞中的mRNA表达，但在相反的其他成员的家庭也在淋巴细胞。此外，还鉴定了另外两个基因，它们不编码具有凝集素样结构域结构的蛋白质，并且似乎被广泛表达。
The myeloid cluster within the natural killer (NK) gene complex comprises several C-type lectin-like receptor genes of diverse and highly important functions in the immune system such as LOX-1 and DECTIN-1. Based on sequences that have become available by whole genome sequencing, we conducted a comparison of the human, chimpanzee, mouse and rat NK gene complex to better characterize this gene family and additional genes of this region in regard of their phylogenetic relationship and evolution within the complex. We found that the arrangement of genes within the primate cluster differs from the order and orientation of the corresponding genes in the rodent complex which can be explained by evolutionary duplication and inversion events. Analysis of individual genes revealed a high sequence conservation supporting the prime importance of the encoded proteins. Expression analyses of the more recently described CLEC12B and CLEC9A genes displayed not only mRNA expression in monocytic and dendritic cells, but in contrast to other members of the family also in lymphocytes. Further, two additional genes were identified, which do not encode proteins with lectin-like domain structure and seem to be widely expressed.