Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF
Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF
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DOI：
10.1093/humrep/deh535
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发表时间：
2004-12-01
影响因子：
6.1
通讯作者：
Matzuk, MM
中科院分区：
文献类型：
--
作者：
McKenzie, LJ;Pangas, SA;Matzuk, MM
背景资料：胚胎发育的生化标志物将通过优化卵母细胞和胚胎选择来增加IVF成功怀孕的机会，并允许移植更少的胚胎。在这项研究中，我们相关的卵丘颗粒细胞基因表达的透明质酸合成酶2（HAS 2），环氧合酶2（COX 2; PTGS 2）和gremlin（GREM 1）与随后的胚胎发育，寻找一个参数的胚胎选择。方法：对8例连续行IVF和ICSI的患者的卵丘细胞基因表达进行前瞻性测定。取卵后立即将卵丘从卵母细胞剥离，并使用一步实时定量RT-PCR测定评估PTGS 2、HAS 2和GREM 1的卵丘基因表达。卵母细胞质量、受精率和胚胎形态与相关基因表达相关。结果：从108个卵母细胞发育成70个胚胎（64.8%受精率）的卵丘细胞的基因表达数据。卵丘PTGS 2、HAS 2和GREM 1的表达在发育为高质量胚胎（3、4和5级）的卵母细胞中高于低质量胚胎（1和2级）（P
Background: A biochemical marker for embryo development would increase the chance of a successful pregnancy with IVF by optimizing oocyte and embryo selection, and allow fewer embryos to be transferred. In this study, we correlated cumulus granulosa cell gene expression of hyaluronic acid synthase 2 (HAS2), cyclooxygenase 2 (COX2; PTGS2) and gremlin (GREM1) with subsequent embryo development in search of a parameter for embryo selection. Methods: Cumulus cell gene expression was determined prospectively on eight consecutive patients undergoing IVF with ICSI. Immediately following oocyte retrieval, the cumulus was stripped from the oocyte, and cumulus gene expression for PTGS2, HAS2 and GREM1 was assessed using a one-step real-time quantitative RT-PCR assay. Oocyte quality, fertilization and embryo morphology were correlated to relative gene expression. Results: Gene expression data were available on cumulus cells from 108 oocytes that developed into 70 embryos (64.8% fertilization rate). Cumulus PTGS2, HAS2 and GREM1 expression was higher from oocytes that developed into higher quality embryos (grades 3, 4 and 5) compared with lower quality embryos (grades 1 and 2) (P