Isolated horizontal cells from carp retina demonstrate dopamine-dependent accumulation of cyclic AMP.
Isolated horizontal cells from carp retina demonstrate dopamine-dependent accumulation of cyclic AMP.
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来自鲤鱼视网膜的分离水平细胞证明了环 AMP 的多巴胺依赖性积累。
DOI：
10.1073/pnas.78.12.7825
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发表时间：
1981
影响因子：
11.1
通讯作者：
Dowling,JE
中科院分区：
文献类型：
--
作者：
VanBuskirk,R;Dowling,JE
在单位重力下，采用酶解和速度沉降的方法将鲤鱼视网膜水平细胞与其他类型视网膜细胞分离。测试了含有水平细胞的分数在各种假定的神经递质存在下积累环AMP的能力。当在3-异丁基-1-甲基黄嘌呤存在的情况下添加微摩尔浓度的多巴胺时，刺激了这些分离细胞中循环AMP的积累。多巴胺拮抗剂如氟哌啶醇、(+)-丁他卡莫和氟非那嗪，可阻断环AMP在完整分离水平细胞中多巴胺依赖的积累。结果表明，鲤鱼水平细胞存在与腺苷酸环化酶[ATP焦磷酸盐裂解酶（环化），EC 4.6.1.1]相关的突触后多巴胺受体。
Horizontal cells of the carp retina were separated from other retinal cell types by using enzymatic dissociation and velocity sedimentation at unit gravity. Fractions containing horizontal cells were tested for their ability to accumulate cyclic AMP in the presence of various putative neurotransmitters. Micromolar concentrations of dopamine, when added in the presence of 3-isobutyl-1-methylxanthine, stimulated cyclic AMP accumulation in these isolated cells. The dopamine-dependent accumulation of cyclic AMP in intact isolated horizontal cells was blocked by nanomolar concentrations of dopamine antagonists such as haloperidol, (+)-butaclamol, and fluphenazine. The results indicate that there is a postsynaptic dopamine receptor on carp horizontal cells that is associated with adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1].