A comparative cytogenetic study of 17 Avena species using Am1 and (GAA)6 oligonucleotide FISH probes
A comparative cytogenetic study of 17 Avena species using Am1 and (GAA)6 oligonucleotide FISH probes
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使用 Am1 和 (GAA)(6) 寡核苷酸 FISH 探针对 17 种燕麦物种进行比较细胞遗传学研究
DOI：
10.1007/s11738-018-2721-9
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发表时间：
2018-08-01
影响因子：
2.6
通讯作者：
Chen, Liang
中科院分区：
文献类型：
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作者：
Luo, Xiaomei;Tinker, Nick A.;Chen, Liang
采用荧光原位杂交（FISH）技术，利用Am1 （oligo-Am1）和（GAA）(6)寡核苷酸探针，研究了17个分类群之间的基因组和物种关系。Oligo-Am1 （51 bp）几乎在C基因组的所有染色体上都有强烈的杂交。在AACC四倍体和AACCDD六倍体中发现了6个A和C基因组易位，在AACCDD六倍体中发现了4个C和D基因组的小易位，在sativa中发现了2个C和D基因组的大易位。在17种猕猴中，（GAA）(6)区主要在A、B、C基因组的中心点周围位置和B基因组的末端处以细长的条带形式出现。然而，在D基因组中未观察到GAA(6)信号位点。AA和CC二倍体的（GAA）(6)信号数不变，但信号强度不同。（GAA）(6)信号模式在AABB、AACC和AACCDD多倍体中存在差异，每种多倍体均表现出一种信号模式。（GAA）(6)信号数在二倍体中是一致的，在多倍体中是不同的，说明了avenena种间的基因组内关系。本研究首次对这两种基于合成重复单位（18-51 bp）的寡核苷酸进行了测试。我们的方法为未来的研究铺平了道路，在这些研究中，FISH探针可以用于将其他具有里程碑意义的基因组序列寡核苷酸分配到物理染色体上。
Fluorescence in situ hybridization (FISH) was performed to explore the genomic and species relationships among 17 taxa using Am1 (oligo-Am1) and (GAA)(6) oligonucleotide probes. Oligo-Am1 (51 bp) hybridized strongly over almost the entire length of all chromosomes in the C genome. Six translocations between the A and C genomes were found in AACC tetraploids and AACCDD hexaploids, four minor translocations between the C and D genomes were found in AACCDD hexaploids, and two large translocations between the C and D genomes were found in A. sativa. In the 17 Avena species, (GAA)(6) regions mainly appear as sharp, thin bands at pericentromeric positions in the A, B, and C genomes and at termini in the B genome. However, no (GAA)(6) signal loci were observed in the D genome. The (GAA)(6) signal number was constant in both AA and CC diploids, though with different signal intensities. The (GAA)(6) signal pattern was diverse in AABB, AACC, and AACCDD polyploids, with each species exhibiting one signal pattern. The (GAA)(6) signal number was consistent in diploids and varied in polyploids, describing an intragenomic relationship among Avena species. This study is the first to test these two oligonucleotides, which are based on synthesized repeat units (18-51 bp), in the genus Avena. Our approach paves the way for future studies in which FISH probes can be used to assign other landmark genomic sequence oligonucleotides to physical chromosomes.