Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses
Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses
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DOI：
10.1128/jcm.01224-15
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发表时间：
2015-08-01
影响因子：
9.4
通讯作者：
Yuen, Kwok-Yung
中科院分区：
文献类型：
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作者：
Chan, Jasper Fuk-Woo;Choi, Garnet Kwan-Yue;Yuen, Kwok-Yung
基于小RNA测序（Seq）数据分析的结果，我们开发了高度灵敏和特异性的实时逆转录（RT）-PCR检测方法，该方法使用锁定核酸探针靶向中东呼吸综合征冠状病毒（MERS-CoV）和其他人类冠状病毒的丰富表达的前导序列。分析和临床评估表明，它们在检测这些冠状病毒方面非劣效于商业多重PCR检测。
Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.