Dibutyl phthalate exposure disrupts the progression of meiotic prophase I by interfering with homologous recombination in fetal mouse oocytes
Dibutyl phthalate exposure disrupts the progression of meiotic prophase I by interfering with homologous recombination in fetal mouse oocytes
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邻苯二甲酸二丁酯暴露通过干扰胎儿小鼠卵母细胞的同源重组来破坏减数分裂前期 I 的进展。
DOI：
10.1016/j.envpol.2019.05.107
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发表时间：
2019-09-01
影响因子：
8.9
通讯作者：
He, Junlin
中科院分区：
文献类型：
--
作者：
Tu, Zhihan;Mu, Xinyi;He, Junlin
邻苯二甲酸二丁酯(DBP)是使用最广泛的增塑剂之一，是一种已知的环境内分泌干扰物，会损害男性和女性的生育能力。本研究对妊娠小鼠在交配后14.5天灌胃给予DBP，连续3天，并在14.5天交配后胎儿卵巢培养中加入DBP，连续3天。孕期DBP暴露干扰了小鼠卵母细胞减数分裂前期I期的进程，特别是从合子线期到粗线期。同时，DBP暴露的粗线期卵母细胞同源重组位点增加，DNA损伤无法修复。此外，DBP通过增加氧化应激导致DNA损伤，降低多种关键减数分裂调节因子的表达，从而诱导卵母细胞凋亡。此外，DBP对减数分裂I前期的影响涉及雌激素受体α和β。总体而言，这些结果显示了暴露于DBP的胎儿卵母细胞的一系列减数分裂缺陷。由于同源重组中的异常可导致非整倍体配子和胚胎，本研究为邻苯二甲酸酯的有害影响提供了新的支持。(C)2019爱思唯尔有限公司。保留所有权利。
Dibutyl phthalate (DBP), one of the most widely used plasticizers, is a known environmental endocrine disruptor that impairs male and female fertility. In this study, oral administration of DBP was given to pregnant mice on 14.5 days post coitus (dpc) for 3 days; and additionally, DBP was added into the culture of 14.5 dpc fetal ovaries for 3 days. DBP exposure during gestation disturbed the progression of meiotic prophase I of mouse oocytes, specifically from the zygotene to pachytene stages. Meanwhile, the DBP-exposed pachytene oocytes showed increased homologous recombination sites and unrepaired DNA damage. Furthermore, DBP caused DNA damage by increasing oxidative stress, decreased the expression of multiple critical meiotic regulators, and consequently induced oocyte apoptosis. Moreover, the effect of DBP on meiosis I prophase involved estrogen receptors alpha and beta. Collectively, these results demonstrated a set of meiotic defects in DBP-exposed fetal oocytes. As aberrations in homologous recombination can result in aneuploid gametes and embryos, this study provides new support for the deleterious effects of phthalates. (C) 2019 Elsevier Ltd. All rights reserved.