Physiological, genomic and transcriptomic comparison of two Brassica napus cultivars with contrasting cadmium tolerance.
Physiological, genomic and transcriptomic comparison of two Brassica napus cultivars with contrasting cadmium tolerance.
复制标题
两个具有对比镉耐受性的甘蓝型油菜品种的生理、基因组和转录组比较。
DOI：
10.1007/s11104-019-04083-0
复制
发表时间：
2019
期刊：
影响因子：
4.9
通讯作者：
Li Nannan
中科院分区：
文献类型：
--
作者：
Wang Shufeng;Sun Juanjuan;Li Shengting;Lu Kun;Meng Hongjun;Xiao Zhongchun;Zhang Zhen;Li Jiana;Luo Feng;Li Nannan
镉（Cd）是危害植物生长最广泛的重金属。甘蓝型油菜（B. napus）是潜在的Cd污染源。而B中Cd从根向地上部的转运机制不明显。甘蓝型油菜对镉毒害的反应尚不清楚。在本研究中，一对B。从39个甘蓝型油菜B中筛选出Cd吸收和根冠转运能力不同的基因型，分别命名为高Cd积累基因型（HC）P78和低Cd积累基因型（LC）P72。不同油菜基因型对Cd的积累特性不同。P78和P72的生理比较表明，P72比P78对Cd的毒性更敏感。通过基因组重测序、转录组学和qRTPCR分析，发现Cd胁迫下P78地上部中BnNramp2; 1和BnNramp4; 2表达显著上调。此外，BnNramp2; 1和BnNramp4; 2可以成功地补充液泡膜定位的镉转运蛋白YCF 1的功能。当BnNramp2; 1和BnNramp4;结果表明，BnNramp 2; 1和. BnNramp 4; 2是促进B根向地上部转运和地上部积累Cd的两种主要的Cd转运蛋白。油菜。
Cadmium (Cd) is the most widespread toxic heavy metal to plant growth. As the second leading oil crop, some genotypes of Brassica napus (B. napus) are potential Cd accumulators. However, the Cd translocation mechanism from root to shoot in B. napus in response to Cd toxicity remains unknown. In the present study, a couple of B. napus genotypes with contrasting Cd uptake and root-toshoot translocation abilities, named P78 (the high Cd accumulator,HC) and P72 (the low Cd accumulator,LC), were chosen from 39 B. napus genotypes with various Cd accumulation features. Physiological comparison of P78 and P72 reveals that P72 is more sensitive to Cd toxicity than P78.With genomic resequencing, transcriptomics and qRTPCR assay, BnNramp2;1 and BnNramp4;2 were focused with highly upregulation in shoot of P78 under Cd treatment condition. Furthermore, BnNramp2;1 and BnNramp4;2 can successfully complement the function of tonoplast localized Cd transporter YCF1. And when BnNramp2;1 and BnNramp4;2 were transferred in Arabidopsis atnramp mutants, the transgenic plants showed better growth rate than mutants under higher Cd stress conditions.The results reveals that BnNramp2;1 and.BnNramp4;2 were two main Cd transporters associated with enhanced root-to-shoot translocation and accumulation of Cd in shoot of B. napus.