STRUCTURAL ORGANIZATION OF POLIOVIRUS RNA REPLICATION IS MEDIATED BY VIRAL-PROTEINS OF THE P2 GENOMIC REGION
STRUCTURAL ORGANIZATION OF POLIOVIRUS RNA REPLICATION IS MEDIATED BY VIRAL-PROTEINS OF THE P2 GENOMIC REGION
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DOI：
10.1128/jvi.64.3.1156-1163.1990
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发表时间：
1990-03-01
影响因子：
5.4
通讯作者：
PASAMONTES, L
中科院分区：
文献类型：
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作者：
BIENZ, K;EGGER, D;PASAMONTES, L
从脊髓灰质炎病毒感染的细胞中分离出与平滑膜小泡结合的转录活性复制复合体。在电子显微镜负染的制剂中，复制复合体呈现为一个不规则形状的长方形结构，附着在几个由病毒诱导的玫瑰花样排列的小泡上。这种制剂的电子显微镜免疫细胞化学表明，脊髓灰质炎病毒复制复合体包含由P2基因组区域编码的膜相关形式的蛋白质(P2蛋白质)。此外，P2蛋白还与病毒RNA相关，它们可以通过紫外线辐射与病毒RNA交联。盐酸胍阻止了P2蛋白的膜结合，但没有改变它们与病毒RNA的联系。这些发现支持这样的结论，即含蛋白2C或2C的前体(S)负责病毒核糖核酸与囊泡膜的附着，并负责复制复合体的空间组织，这是病毒转录正常发挥功能所必需的。提出了病毒复制复合体的结构以及含2C的P2蛋白(S)和囊泡膜的功能模型。
Transcriptionally active replication complexes bound to smooth membrane vesicles were isolated from poliovirus-infected cells. In electron microscopic negatively stained preparations, the replication complex appeared as an irregularly shaped, oblong structure attached to several virus-induced vesicles of a rosettelike arrangement. Electron microscopic immunocytochemistry of such preparations demonstrated that the poliovirus replication complex contains the proteins coded by the P2 genomic region (P2 proteins) in a membrane-associated form. In addition, the P2 proteins are also associated with viral RNA, and they can be cross-linked to viral RNA by UV irridation. Guanidine hydorchloride prevented the P2 proteins from becoming membrane bound but did not change their association with viral RNA. The findings allow the conclusion that the protein 2C or 2C-containing precursor(s) is responsible for the attachment of the viral RNA to the vesicular membrane and for the spatial organization of the replication complex necessary for its proper functioning in viral transcription. A model for the structure of the viral replication complex and for the function of the 2C-containing P2 protein(s) and the vesicular membranes is proposed.