Annulate lamellae and lytic HAV infection in vitro
Annulate lamellae and lytic HAV infection in vitro
复制标题
DOI：
10.1016/s0040-8166(96)80008-5
复制
发表时间：
1996-04-01
期刊：
影响因子：
2.6
通讯作者：
Anderson, DA
中科院分区：
文献类型：
--
作者：
Marshall, JA;Borg, J;Anderson, DA
为探讨甲型肝炎病毒(HAV)裂解株感染BS-C-1细胞与环状病毒(AL)产生的关系，用定量和定性的电子显微镜观察了AL的大小和数量。甲型肝炎病毒裂解株感染BS-C-1细胞的过程分为两个阶段，在第一阶段，细胞增殖和细胞死亡与模拟感染对照组大致相同，但感染细胞中甲型肝炎抗原的数量随着时间的推移而增加，第二阶段细胞分裂最小，细胞死亡明显，感染和对照细胞中均可检测到AL。定量分析表明，感染细胞中AL的平均数量在第1期高于对照细胞；在感染细胞中，处于第1期的AL数量多于第2期；感染细胞中膜质叶/AL的平均数量比对照细胞多。定量分析还表明，AL非常罕见，每个对照细胞只有大约3个AL，每个整个感染细胞只有8个AL。本研究清楚地证实了病毒感染可以刺激AL的产生。结果提示，病毒感染细胞中AL的产生与病毒抗原的合成有关。超微结构观察表明，AL既可来自粗面内质网，也可来自核膜。
The aim of this study was to examine the relationship between viral infection and annulate lamellae (AL) production by using quantitative and qualitative electron microscopy to document the size and numbers of AL in BS-C-1 cells infected with a lytic strain of hepatitis A virus (HAV), The progress of the HAV infection was found to occur in two phases, In phase 1, cell proliferation and cell death were roughly the same as that of the mock infected control, but there was an increase with time in the amount of hepatitis A antigen in the infected cells, In phase 2 cell division was minimal and cell death became manifest, AL were detected in both infected and control cells, Quantitative analysis indicated that the average number of AL was greater in infected cells compared to that in control cells in phase 1; in infected cells there were greater numbers of AL in phase 1 than in phase 2; the average number of membraneous leaves/AL was greater in infected cells than in control cells, Quantitative analysis also indicated that AL were very rare, with only about three AL per entire control cell and eight AL per entire infected cell, The study clearly establishes that viral infection can stimulate AL production. The data suggest stimulation of AL production in the virus infected cells was linked to the synthesis of viral antigen, Ultrastructural observations indicated that AL could be derived from either the rough endoplasmic reticulum or the nuclear membrane.