Accumulation of human α-synuclein in different cytoskeletons in Lewy bodies in brains of dementia with Lewy bodies
Accumulation of human α-synuclein in different cytoskeletons in Lewy bodies in brains of dementia with Lewy bodies
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DOI：
10.1016/s0304-3940(00)01316-1
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发表时间：
2000-08-18
影响因子：
2.5
通讯作者：
Kosaka, K
中科院分区：
文献类型：
--
作者：
Iseki, E;Marui, W;Kosaka, K
我们用免疫组织化学和免疫电镜检查了路易体痴呆（DLB）脑中α -突触核蛋白免疫反应成分的来源，其中α -突触核蛋白阳性LB光镜下由磷酸化的神经丝（PN）阳性LB、微管蛋白阳性LB和两种染色均阴性的LB组成。免疫电镜下，pn阳性LB由pn阳性和α -突触核蛋白阳性的丝状成分组成，提示这些丝状成分来源于部分免疫反应性降低和α -突触核蛋白积累的神经纤维。然而，微管蛋白阳性的LB由微管蛋白阳性和α -突触核蛋白阳性的管状成分组成，这表明这些管状成分来源于弥散性免疫反应性降低和α -突触核蛋白积累的微管。本研究的结果表明，α -突触核蛋白在DLB脑的不同细胞骨架中积累，可能是由于轴突运输受阻。(C) 2000出版的爱思唯尔科学爱尔兰有限公司。
We investigated the origin of alpha-synuclein-immunoreactive components in Lewy bodies (LB) in brains of dementia with Lewy bodies (DLB) using immunohistochemistry and immunoelectron microscopy with anti-alpha-synuclein antibodies and anti-cytoskeleton antibodies, alpha-Synuclein-positive LB light microscopically consisted of phosphorylated neurofilament(PN)-positive LB, tubulin-positive LB and LB that were negative for both stains. Immunoelectron microscopically, PN-positive LB were com posed of PN-positive and alpha-synuclein-positive filamentous components, suggesting that these filamentous components originate from neurofilaments with partially reduced immunoreactivity and alpha-synuclein accumulation. However, tubulin-positive LB were composed of tubulin-positive and alpha-synuclein-positive tubular components, suggesting that these tubular components originate from microtubules with diffusely reduced immunoreactivity and alpha-synuclein accumulation. The results of the present study suggest that alpha-synuclein accumulates in different cytoskeletons in the LB in DLB brains presumably due to a blockage of axonal transport. (C) 2000 Published by Elsevier Science Ireland Ltd.