Content and characterization of plasminogen activators in human lung tumors and normal lung tissue.
Content and characterization of plasminogen activators in human lung tumors and normal lung tissue.
复制标题
人肺肿瘤和正常肺组织中纤溶酶原激活剂的含量和特征。
作者：
G. Markus;H. Takita;S. Camiolo;J. Corasanti;J. Evers;G. Hobika
已经定量测定了手术切除的肺癌以及邻近正常肺组织的纤溶酶原激活物含量。通过对Nagy等人描述的技术进行改进，确定了定量提取的最佳条件。J.癌症，19:614-620,1977)，使用非离子洗涤剂Triton X-100的缓冲溶液。肿瘤中纤溶酶原激活物显著升高（比正常肺高2.5- 4.3倍，取决于样本选择和其他标准），尽管肿瘤之间的个体差异非常大。肿瘤组织病理学特征与活化剂含量、侵袭性与活化剂含量无显著相关性。用兔抗体对纯化的尿激酶作为激活剂作用的抑制剂，发现肺肿瘤含有尿激酶样酶作为主要的纤溶酶原激活剂，而邻近的正常肺组织的激活剂含量由一些尿激酶样酶组成，但主要是不被抗体抑制的酶。通过结合两种亲和层析程序，从肺肿瘤中纯化了尿激酶样激活剂约20,000倍，并与纯化的分子量为55,000的尿激酶进行了比较，肺肿瘤激活剂与尿激酶相同。
The plasminogen activator content of surgically removed lung cancers, as well as that of adjacent normal lung tissue has been determined quantitatively. Optimum conditions for the quantitative extraction have been worked out using a modification of the technique described by Nagy et al. (Int. J. Cancer, 19:614-620, 1977) which utilizes a buffered solution of the non-ionic detergent Triton X-100. Plasminogen activator was significantly elevated in the tumors (2.5- to 4.3-fold over the normal lung, depending on sample selection and other criteria), although individual variations between tumors were extremely large. No significant correlation was found between the histopathological character of the tumors and the activator content, or between invasiveness and activator content. Using rabbit antibody formed against purified urokinase as an inhibitor of activator action, it was found that lung tumors contain a urokinase-like enzyme as the predominant plasminogen activator, while the activator content of the adjacent normal lung tissue consists of some urokinase-like enzyme, but mostly of an enzyme which is not inhibited by the antibody. The urokinase-like activator has been purified approximately 20,000-fold from lung tumors by the combination of two affinity chromatographic procedures, and was compared with purified urokinase with a molecular weight of 55,000 on all criteria used, the lung tumor activator was identical to urokinase.