Determination of carbonyl compounds in the atmosphere by DNPH derivatization and LC-ESI-MS/MS detection
Determination of carbonyl compounds in the atmosphere by DNPH derivatization and LC-ESI-MS/MS detection
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DOI：
10.1016/j.talanta.2006.11.018
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发表时间：
2007-04-30
期刊：
影响因子：
6.1
通讯作者：
Fu, Jiamo
中科院分区：
文献类型：
--
作者：
Chi, Yuguang;Feng, Yanli;Fu, Jiamo
建立了2，4-二硝基苯肼（DNPH）衍生化-高效液相色谱（HPLC）-电喷雾串联质谱（ESI/MS）测定32种羰基化合物的方法，并成功应用于广州市荔湾区某居民区（S1）和天河区某研究所（S2）大气样品中32种羰基化合物的测定。优化了负离子模式下的电喷雾电离质谱（ESI-MS/MS）的操作条件，包括母离子和子离子的选择、去簇电位（DP）、入口电位（EP）、碰撞能（CE）、碰撞池出口电位（CXP）以及缓冲液对ESI-MS/MS过程的影响。校准曲线的回归系数（R-2）、回收率、重现性（R.S.D.，方法的检出限（LOD）分别为0.9938-0.9999、90- 104%、1.7- 11%和0.4- 9.4ng/m3。在大多数样品中，丙酮是最丰富的羰基在两个采样点，甲醛，乙醛和丁醛/2-丁醛也是丰富的羰基。与LC-UV方法相比，LC-ESI-MS/MS提高了LOD、一些共洗脱化合物的分离和精密度（主要是对高分子量羰基化合物）。（c）2006 Elsevier B. V.保留所有权利。
A method of determination of 32 carbonyl compounds by high performance liquid chromatography (HPLC) and electrospray ionization (ESI) tandem mass spectrometry (MS/MS) after derivatization with 2,4-dinitrophenylhydrazine (DNPH) was developed and successfully applied to the atmosphere sample of a residential area of Liwan District (S1) and a research institute of Tianhe District (S2) in Guangzhou, China. Some operation conditions of ESI-MS/MS in the negative ion mode including selection of parent and daughter ions, declustering potential (DP), entrance potential (EP), collision energy (CE), collision cell exit potential (CXP) and effect of buffer in ESI-MS/MS process were optimized. The regression coefficient of the calibration curves (R-2), recovery, reproducibility (R.S.D., n = 5) and limit of detection (LOD) were in the range of 0.9938-0.9999, 90-104%, 1.7-11 % and 0.4-9.4 ng/m(3) respectively. Among most of the samples, acetone was the most abundant carbonyl in two sampling sites and formaldehyde, acetaldehyde and butyral dehyde/2-butan one were also abundant carbonyls. In contrast to LC-UV method, the LOD, the separation of some co-eluting compounds and the precision (mainly to higher molecular weight carbonyls) are all improved by LC-ESI-MS/MS. (c) 2006 Elsevier B.V. All rights reserved.