The pretreatment of softwood chips and pellets by alkali sulfonation and thermomechanical pulping and its influence on lignin redistribution and the enzyme mediated hydrolysis of the cellulose component
The pretreatment of softwood chips and pellets by alkali sulfonation and thermomechanical pulping and its influence on lignin redistribution and the enzyme mediated hydrolysis of the cellulose component
复制标题
碱磺化和热机械制浆对针叶木片和颗粒的预处理及其对木质素再分布和纤维素组分酶介导水解的影响
DOI：
10.1039/d2se00798c
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Saddler Jack
中科院分区：
文献类型：
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作者：
Takada Masatsugu;Wu Jie;Del Rio Pablo;Kawamoto Haruo;Saddler Jack
碱磺化，然后热机械处理的软木片和丸成功地提高了酶介导的水解纤维素组分的酸基团的木质素组分上的掺入。虽然蒸汽处理（ST）比液体热水（LHW）预处理木屑更有效，相比之下，LHW预处理的颗粒更好。结果表明，通过SEM和UV显微镜，预处理芯片在中间层分层。蒸汽处理在次生壁内产生间隙，可能是由于快速压力释放导致纤维素对酶的可及性增强。与此相反，广泛的破坏细胞壁，而不是分层发生在小球预处理ST和LHW。这可能是由于木质素的更浓缩的结构。然而，这两种预处理导致木质素重新分布和细胞壁结构的变化，这增强了酶介导的纤维素组分的水解。
Alkaline sulfonation followed by thermomechanical treatment of softwood chips and pellets successfully improved the enzyme-mediated hydrolysis of the cellulose component by the incorporation of acid groups onto the lignin component. Although steam treatment (ST) was more effective than liquid hot water (LHW) pretreatment of wood chips, in contrast, the LHW pretreatment of pellets was better. It was shown, via SEM and UV microscopy, that pretreated chips were delaminated at the middle lamella. Steam treatment produced gaps within the secondary wall, likely due to the rapid pressure release resulting in enhanced accessibility of the cellulose to the enzymes. In contrast, extensive destruction of the cell wall rather than delamination occurred in pellets pretreated by both ST and LHW. This was likely due to the lignins' more condensed structure. However, both pretreatments resulted in lignin redistribution and changes to the cell wall structure, which enhanced the enzyme-mediated hydrolysis of the cellulosic component.