Glycopolymers Mimicking GM1 Gangliosides: Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition
Glycopolymers Mimicking GM1 Gangliosides: Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition
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DOI：
10.1002/asia.201900053
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发表时间：
2019-04-01
影响因子：
4.1
通讯作者：
Miura, Yoshiko
中科院分区：
文献类型：
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作者：
Terada, Yuhei;Hoshino, Yu;Miura, Yoshiko
通过将多种类型的碳水化合物并入聚合物主链中来合成模拟GM1神经节苷脂的糖共聚物。将糖聚合物固定在金表面上，并使用表面等离子体共振成像分析与霍乱毒素B亚基（CT B）的相互作用。含有半乳糖和神经氨酸的糖共聚物显示出对CTB的增强识别。增强的相互作用，主要是因为在解离过程中的GM1结合口袋中的神经氨酸基团的结合的改善。半乳糖和神经氨酸的这种协同性是通过掺入到相同的柔性聚合物主链中来实现的，并且揭示了半乳糖和神经氨酸基团的紧密放置的重要性。这些结果在医学领域和生物功能材料的开发中具有重要的应用价值。
Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.