SYNAPTONEMAL COMPLEXES IN FEMALE AND MALE MEIOTIC PROPHASE OF EPHESTIA-KUEHNIELLA (LEPIDOPTERA)
SYNAPTONEMAL COMPLEXES IN FEMALE AND MALE MEIOTIC PROPHASE OF EPHESTIA-KUEHNIELLA (LEPIDOPTERA)
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DOI：
10.1038/hdy.1993.154
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发表时间：
1993-10-01
期刊：
影响因子：
3.8
通讯作者：
TRAUT, W
中科院分区：
文献类型：
--
作者：
MAREC, F;TRAUT, W
应用微铺展技术和电子显微镜研究了地中海麦蛾Ephestia kuehniella卵母细胞和精母细胞粗线期核联会复合体（SC）的组成。在两种性别中，三个常染色体SC可以确定其形态标志，一个SC有一个突出的旋钮和两个SC有核仁组织者区域。在卵母细胞中SC发育的后期，两个核仁SC显示侧元件的电子致密末端修饰。在一些卵母细胞中，性染色体二价体是不显眼的，但在大多数情况下，它被识别的异染色质缠结与W染色体，较短的W染色体轴和/或不完全配对。雄性联会复合体与雌性联会复合体相比，含有重组结节。几乎每个二价体都与一个RN相关，很少没有或两个RN。沿着SC，RN优先位于远端位置。RN在雄性SC中的存在和它们在雌性SC中的缺失对应于在雄性减数分裂中的交换和交叉的存在和它们在雌性减数分裂中的缺失。SC补体的平均总长度是男性比女性，但在两种性别，变化是相当大的。我们假设，男性的SC变得更长的粗线期阶段的进展，而在女性的SC的一段时间的延长是由一段时间的缩短。
Synaptonemal complex (SC) complements of oocyte and spermatocyte pachytene nuclei in the Mediterranean flour moth, Ephestia kuehniella, were investigated using a microspreading technique and electron microscopy. In both sexes, three autosomal SCs could be identified by their morphological landmarks; one SC has a prominent knob and two SCs have nucleolus organizer regions. In a later stage of SC development in oocytes, the two nucleolar SCs displayed electron dense terminal modifications of the lateral elements. In some oocytes, the sex chromosome bivalent was inconspicuous but in the majority it was recognized by heterochromatin tangles associated with the W chromosome, a shorter W chromosome axis and/or incomplete pairing. Male synaptonemal complexes in contrast to female ones contained recombination nodules (RNs). Almost every bivalent was associated with one RN, rarely none or two RNs. Along the SCs, RNs were preferentially located in distal positions. The presence of RNs in male SCs and their absence in female SCs corresponds with the presence of crossing over and chiasmata in male meiosis and their absence in female meiosis. The mean total length of SC complements was greater in males than in females, but in both sexes, variation was considerable. We assume that male SCs become longer with progression of the pachytene stage while in female SCs a period of elongation is followed by a period of shortening.