Quantitation and characterization of phospholipids in pharmaceutical formulations by liquid chromatography-mass spectrometry
Quantitation and characterization of phospholipids in pharmaceutical formulations by liquid chromatography-mass spectrometry
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DOI：
10.1016/s0021-9673(00)00148-5
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发表时间：
2000-04-21
影响因子：
4.1
通讯作者：
Dupuis, S
中科院分区：
文献类型：
--
作者：
Carrier, A;Parent, J;Dupuis, S
建立了一种简便、快速的磷脂分析方法--液相色谱-质谱联用技术。在硅胶柱上使用氯仿-10 mM乙酸铵-甲醇（30：5：65）的线性梯度混合物实现磷脂分子种类的分离。质谱条件的优化允许该方法分离和检测主要作为质子化分子种类的磷脂。与现有的LC-MS方法相比，实现了总分析时间和灵敏度的改进。所有主要的磷脂分子类的分离，在不到6分钟内实现。观察到显着的改善，在线性的响应的磷脂研究提供了超过三个数量级的线性响应。还提供了支持该方法用于表征主要磷脂分子种类的验证数据。(C)2000 Elsevier Science B. V.保留所有权利。
A simple and fast method for phospholipid analysis was developed using high-performance liquid chromatography-mass spectrometry with an atmospheric pressure ionization interface. Separation of the phospholipid molecular species was achieved using a linear gradient of a mixture of chloroform-10 mM ammonium acetate-methanol (30:5:65) on a silica column. Optimization of the mass spectrometer conditions has allowed the method to separate and detect the phospholipids mainly as protonated molecular species. In comparison to existing LC-MS methods, improvement in the total analysis time and sensitivity were achieved. Separation of all major phospholipid molecular classes was achieved in less than 6 min. Marked improvement was observed in the linearity of the response of the phospholipids studied providing a linear response over three orders of magnitude. Data supporting the validation of this method for the characterization of major phospholipids molecular species are also presented. (C) 2000 Elsevier Science B.V. All rights reserved.