CLONING OF HUMAN THYROGLOBULIN COMPLEMENTARY-DNA
CLONING OF HUMAN THYROGLOBULIN COMPLEMENTARY-DNA
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DOI：
10.1016/0014-5793(82)80346-3
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发表时间：
1982-01-01
期刊：
影响因子：
3.5
通讯作者：
VASSART, G
中科院分区：
文献类型：
--
作者：
BROCAS, H;CHRISTOPHE, D;VASSART, G
The biosynthetic precursor of thyroid hormones, thyroglobulin (Tg) is a dimer of Mr 300 000 subunits translated from an 8000 base mRNA [l]. Congenital defects of Tg gene expression have been described in animals and in man leading to goiter and hypothyroidism [2]. Characterization of these defects at the molecular level will undoubtedly lead to a better understanding of the mechanisms involved in normal thyroid cell function. While such studies are underway in an animal model [3], the lack of a suitable probe has hindered a similar approach to the human cases. Here, we describe the isolation of human thyroglobulin (h Tg) mRNA and the cloning in Escherichia coli of cDNA fragments representing 41% of the structural gene. A preliminary account of this study has been presented [4].
