Ultraviolet radiation regulates cortisol activity in a waveband-dependent manner in human skin ex vivo.
Ultraviolet radiation regulates cortisol activity in a waveband-dependent manner in human skin ex vivo.
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DOI：
10.1111/bjd.12096
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发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Slominski AT
中科院分区：
文献类型：
--
作者：
Skobowiat C;Sayre RM;Dowdy JC;Slominski AT
11β-hydroxysteroid dehydrogenase type 1 (HSD11B1), 11β-hydroxysteroid dehydrogenase type 2 (HSD11B2), glucocorticoids (GC) and their receptor (GR) play a key role in tissue-specific regulation of GC action. The expression of genes coding HSD11B1, HSD11B2 and GR and their protein products, and levels of cortisol were determined in human skin explants and/or co-cultured keratinocytes/melanocytes after treatment with ultraviolet A (UVA), B (UVB) or C (UVC) wavebands. Skin from foreskins and/or co-cultured human keratinocytes/melanocytes were irradiated with UVA, UVB or UVC (skin) and incubated for 12 and 24 h. Methods of RT-PCR, western blot (WB), ELISA and immunohistochemistry (IHC) were used to determine expression and localization of corresponding genes or antigens, respectively. UVB enhanced the HSD11B1 gene and protein expression in a dose dependent manner, while UVA was without effect. Similarly, UVC increased HSD11B1 protein product as measured by IHC. UVB and UVC enhanced cortisol production and decreased epidermal GR expression with UVA having no detectable effects. Although both UVA and UVB stimulated HSD11B2 gene expression, only UVA increased HSD11B2 protein product levels with UVB and UVC being without an effect. We suggest that these differential, waveband dependent effects of UVR on cutaneous HSD11B1, HSD11B2, GR gene and corresponding protein products expression and cortisol production are both to protect and/or restore the epidermal barrier homeostasis against disruption caused by elevated cortisol level induced by UVB and UVC.
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DOI：
10.1152/ajpendo.00519.2004
发表时间：
2005-04-01
影响因子：
5.1
作者：
Slominski, A;Zbytek, B;Wortsman, J
通讯作者：
Wortsman, J
DOI：
10.1111/j.1749-6632.2010.05902.x
发表时间：
2011-01-01
期刊：
TRENDS IN NEUROENDOCRINOLOGY
影响因子：
--
作者：
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通讯作者：
Moisan, Marie-Pierre
影响因子：
4.1
作者：
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通讯作者：
Paus, Ralf
DOI：
10.1016/j.bbrc.2010.11.059
发表时间：
2011-01-07
影响因子：
3.1
作者：
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通讯作者：
Philpott, Michael P.
影响因子：
4.1
作者：
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通讯作者：
Buttgereit, Frank