Replication of herpes simplex virus DNA: localization of replication recognition signals within defective virus genomes.
Replication of herpes simplex virus DNA: localization of replication recognition signals within defective virus genomes.
复制标题
单纯疱疹病毒 DNA 的复制：复制识别信号在有缺陷的病毒基因组内的定位。
DOI：
10.1073/pnas.78.2.742
复制
发表时间：
1981
影响因子：
11.1
通讯作者：
Frenkel,N
中科院分区：
文献类型：
--
作者：
Vlazny,DA;Frenkel,N
序列传代1型单纯疱疹病毒（HSV-1）毒株Justin先前已被证明含有缺陷病毒基因组，由源自标准病毒DNA S组分末端的序列从头到尾的重复组成。将纯化的单缺陷基因组重复单元与培养辅助病毒DNA共转染到兔皮肤细胞上，成功地实现了串联缺陷DNA分子的再生和复制。因此，有缺陷的HSV-1 （Justin）基因组在其有限的DNA序列中包含HSV DNA复制和包装所需的一组足够的识别位点。再生缺陷病毒基因组内重复单元的排列与它们通过滚动循环机制复制一致，其中单个重复单元作为循环模板。这种复制最活跃地发生在感染后的后期，并且可以被低浓度的磷酸盐醋酸酯所抑制，这种磷酸盐醋酸酯可以选择性地抑制单纯疱疹病毒特异性的病毒DNA聚合酶。结果显示，所得到的串联体被切割成Mr 100 X 10(6)的DNA分子，该分子一端终止于亲本标准病毒DNA的适当交流端序列。
Serially passaged herpes simplex virus type 1 (HSV-1) strain Justin was previously shown to contain defective virus genomes consisting of head-to-tail reiterations of sequences derived from the end of the S component of the standard virus DNA. Cotransfection of purified monomeric defective genome repeat units with foster helper virus DNAs onto rabbit skin cells resulted in regeneration and replication of concatemeric defective DNA molecules which were successfully encapsidated. Thus, defective HSV-1 (Justin) genomes contain, within their limited DNA sequences, a sufficient set of recognition sites required for HSV DNA replication and packaging. The arrangement of repeat units within the regenerated defective virus genomes was consistent with their replication by a rolling circle mechanism in which a single repeat unit served as the circularized template. This replication occurred most actively late after infection and could be shown to be inhibited by low concentrations of phosphonoacetate known to inhibit the HSV-specified viral DNA polymerase selectively. The resultant concatemers were shown to be cleaved to Mr 100 X 10(6) DNA molecules which were terminated at one end with the proper ac end sequence of the parental standard virus DNA.