Biomimetic Approach to Stimulate Osteogenesis on Titanium Implant Surfaces Using Fibronectin Derived Oligopeptide.
Biomimetic Approach to Stimulate Osteogenesis on Titanium Implant Surfaces Using Fibronectin Derived Oligopeptide.
复制标题
使用纤连蛋白衍生寡肽刺激钛植入物表面成骨的仿生方法。
DOI：
10.2174/1381612822666160203143053
复制
发表时间：
2016
影响因子：
3.1
通讯作者：
Y. Ku
中科院分区：
文献类型：
--
作者：
Young‐Dan Cho;Sung;H. Bae;Won;K. Kim;H. Ryoo;Y. Seol;Yong;I. Rhyu;Y. Ku
我们以前的研究表明，重组纤维连接蛋白（FN）衍生的寡肽，我们命名为F20刺激成骨细胞的粘附，增殖和分化在体外和体内。在本研究中，我们使用一种合成的寡肽，并研究了钛盘上的F20涂层的成骨潜力，以刺激牙种植体表面改性的上级骨结合。利用共聚焦激光扫描显微镜（CLSM）观察钛的表面特征。通过吸附程序将合成F20涂覆到机加工或SLA钛盘上。将ST2细胞接种在钛盘上。用扫描电镜和激光共聚焦显微镜观察细胞粘附，用picogreen法检测细胞增殖，用实时荧光定量PCR、ALP活性测定、免疫印迹法和ALP染色法检测成骨细胞分化。通过荧光检测盘上的FITC标记的F20涂层，显示出良好的F20吸附和根据表面粗糙度的不同涂层图案。在SEM和CLSM观察中，细胞很好地附着在机加工表面上，并且在涂覆有F20的盘上比在其他盘上看到更大的应力纤维形成。F20通过细胞外信号调节激酶（Erk）信号通路刺激细胞增殖以及成骨细胞分化。这些细胞对F20的反应在机械加工的钛表面上比SLA表面稍好。这些结果表明，F20通过Erk途径促进骨生成，是一种合适的生物分子，用于牙科植入物的表面改性，以改善骨整合。
Our previous studies demonstrated that a recombinant fibronectin (FN)-derived oligopeptide that we named F20 stimulated osteoblast adhesion, proliferation, and differentiation in vitro and in vivo. In the present study, we used a synthetic oligopeptide and investigated the osteogenic potential of F20 coating on titanium discs, to stimulate superior osseointegration for dental implant surface modification. Surface characteristic analysis of titanium was performed by confocal laser scanning microscopy (CLSM) observation. Synthetic F20 was coated onto the machined or SLA titanium discs by an adsorption procedure. ST2 cells were seeded on the titanium discs. We evaluated cell adhesion with SEM and CLSM observation, cell proliferation with picogreen assay, and osteoblast differentiation with real-time PCR, ALP activity assay, immunoblot assay and ALP staining. FITC-labeled F20 coating on the discs was detected by fluorescence, showing good F20 adsorption and different coating patterns according to the surface roughness. In the SEM and CLSM observations, cells were well attached on the machined surface and greater stress fiber formation was seen on discs coated with F20 than on other discs. F20 stimulated cellular proliferation, as well as osteoblast differentiation through the extracellular signalregulated kinase (Erk) signaling pathway. These cellular responses to F20 were slightly better on the machined titanium surface than the SLA surface. These results suggest that F20 promotes osteogenesis through the Erk pathway and is a suitable biomolecule for surface modification of dental implants for improved osseointegration.