Cell death during preoviposition period in Boophilus microplus tick
Cell death during preoviposition period in Boophilus microplus tick
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DOI：
10.1016/j.vetpar.2006.10.017
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发表时间：
2007-03-31
影响因子：
2.6
通讯作者：
Masuda, A.
中科院分区：
文献类型：
--
作者：
Freitas, D. R. J.;Rosa, R. M.;Masuda, A.
程序性细胞死亡（PCD）存在于多细胞生物的发育过程中，从胚胎发生到死亡。在微小牛蜱的雌性中，从宿主脱离后，几个器官的质量减少。为了更好地表征消除不必要组织的细胞死亡过程，使用琼脂糖凝胶中的DNA片段化、彗星和TUNEL测定以及通过半胱天冬酶测定的细胞凋亡活化途径研究唾液腺、卵巢和突触神经节的变性。从宿主体内清除蜱虫后，在48和72小时，在唾液腺和卵巢中观察到DNA片段化和caspase-3的酶活性;在突触中，这些参数在48小时后维持在低水平。获得的结果表明，有一个精细的控制组织维护通过细胞凋亡。(c)2006 Elsevier B. V.保留所有权利。
Programmed cell death (PCD) is present during the development of multicellular organisms and occurs from embryogenesis to death. In females of Boophilus microplus, the mass of several organs is reduced after the detachment from the host. In order to better characterize the cell death process that eliminates unnecessary tissues, the degeneration of salivary glands, ovaries and synganglia was investigated using DNA fragmentation in agarose gel, comet and TUNEL assays, and apoptosis activation pathway by the caspase assay. DNA fragmentation and enzymatic activity of caspase-3 were observed in salivary glands and ovaries at 48 and 72 It after tick removal from the host; in synganglia these parameters were maintained at low levels upon 48 h. The results obtained suggest that there is a refined control of tissue maintenance through apoptosis. (c) 2006 Elsevier B.V. All rights reserved.