Molecular testing on the hybrid-origin hypothesis of an alpine serpentine plant Saxifraga yuparensis (sect. Bronchiales, Saxifragaceae) endemic to Mt. Yubari, Hokkaido, Japan.
Molecular testing on the hybrid-origin hypothesis of an alpine serpentine plant Saxifraga yuparensis (sect. Bronchiales, Saxifragaceae) endemic to Mt. Yubari, Hokkaido, Japan.
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日本北海道夕张山特有的高山蛇纹石植物虎耳草（支气管目，虎耳草科）杂交起源假说的分子测试。
DOI：
10.11646/phytotaxa.373.1.2
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发表时间：
2018
期刊：
影响因子：
1.1
通讯作者：
Nakamura K.
中科院分区：
文献类型：
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作者：
Tamura S.;Fukuda T.;Pimenova E.A.;Petrunenko E.A.;Krestov P.V.;Chernyagina O. A.;Tsuboi H.;Takahashi H.;Nishikawa Y.;Shimamura T.;Fujita H.;Nakamura K.
田村沙弥、福田智子、埃琳娜·A.叶卡捷琳娜·皮梅诺娃斯韦特兰娜·彼得鲁年科奥尔加·邦达丘克Chernyagina，YOSHIHISA SUYAMA，YOSHIHIRO TSUNAMOTO，AYUMU MATSUO，HAYATO TSUBOI，HIDEKI TAKAHASHI，KEN SATO，YOKO NISHIKAWA，TAKASHI SHIMAMURA，HIROKO FUJITA & KOH中村一种高山植物虎耳草（Saxifraga yuparensis）是由Mt.夕张是日本北海道的一个新种，根据形态中介性，它被认为是由同一组的两个种即S. nishidae，一个二倍体物种特有的附近悬崖组成的绿片岩和四倍体S。rebunshirensis在日本和俄罗斯远东地区分布较广。虎耳草yuparensis是红名单，这是至关重要的保护规划，以澄清这是否是一个直接的杂交，缺乏一个独特的基因库。通过分子和细胞学数据对直接杂交假说进行了检验。在核核糖体和叶绿体DNA树的基础上最大简约和贝叶斯标准，S。yuparensis和S. rebunshirensis与其他几个同源群形成一支，而S. nishidae形成了另一个不同的分支。全基因组SNP数据在主坐标空间中清楚地将这三个物种分开，将S。yuparensis不介于S. rebunshirensis和S.鱼科。染色体观察表明，S.玉兰油是四倍体，而不是二倍体-四倍体杂交直接产生的三倍体。此外，植物标本的观察表明，S。yuparensis属于S. rebunshirensis。这些结果表明S. yuparensis不是S. rebunshirensis和S.但它是一个独特的谱系和非常狭窄的特有种，值得加强保护。
SAYA TAMURA, TOMOKO FUKUDA, ELENA A. PIMENOVA, EKATERINA A. PETRUNENKO, PAVEL V. KRESTOV, SVETLANA N. BONDARCHUK, OLGA A. CHERNYAGINA, YOSHIHISA SUYAMA, YOSHIHIRO TSUNAMOTO, AYUMU MATSUO, HAYATO TSUBOI, HIDEKI TAKAHASHI, KEN SATO, YOKO NISHIKAWA, TAKASHI SHIMAMURA, HIROKO FUJITA & KOH NAKAMURA An alpine plant Saxifraga yuparensis is endemic to a scree consisting of greenschist of Mt. Yubari in Hokkaido, Japan and it has been proposed as an immediate hybrid derived from two species of the same section Bronchiales based on morphological intermediacy: namely S. nishidae, a diploid species endemic to a nearby cliff composed of greenschist and tetraploid S. rebunshirensis comparatively broadly distributed in Japan and Russian Far East. Saxifraga yuparensis is red-listed and it is crucial for conservation planning to clarify whether this is an immediate hybrid and lacks a unique gene pool. The immediate-hybrid hypothesis was tested by molecular and cytological data. In nuclear ribosomal and chloroplast DNA trees based on maximum parsimony and Bayesian criteria, S. yuparensis and S. rebunshirensis formed a clade with several other congeners while S. nishidae formed another distinct clade. Genome-wide SNP data clearly separated these three species in principal coordinate space, placing S. yuparensis not in-between of S. rebunshirensis and S. nishidae. Chromosome observation indicated that S. yuparensis is tetraploid, not triploid directly derived from diploid-tetraploid crossing. Additionally, observation of herbarium specimens revealed that leaf apex shape of S. yuparensis fell within the variation of S. rebunshirensis. These results indicate that S. yuparensis is not an immediate hybrid of S. rebunshirensis and S. nishidae but a distinct lineage and an extremely narrow endemic species, that deserves for intensive conservation.