Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: Plant-dependent enrichment and seasonal shifts revealed
Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: Plant-dependent enrichment and seasonal shifts revealed
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DOI：
10.1128/aem.67.10.4742-4751.2001
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发表时间：
2001-10-01
影响因子：
4.4
通讯作者：
Berg, G
中科院分区：
文献类型：
--
作者：
Smalla, K;Wieland, G;Berg, G
对病原真菌黄萎病、草莓、油菜和马铃薯3种寄主植物的根际细菌群落进行了分析。我们的目标是确定根际效应依赖于植物的程度，以及连续两年种植同样的作物是否会增加这种效应。在植被期间采集了五次根际或土壤样本。为了进行与栽培无关的分析，从从根部或土壤样本中回收的微生物颗粒中提取总群落DNA。从土壤或根际细菌DNA中扩增出16S rDNA片段，进行变性梯度凝胶电泳(DGGE)分析。DGGE指纹图谱显示，根际细菌种群的相对丰度随着植物的变化而变化，在第二年变得更加明显。油菜和马铃薯根际群落的DGGE格局比草莓群落的DGGE更相似。在这两年中，观察到细菌根际种群丰度和组成的季节性变化。与植物物种无关，两年的第一次采样时间的模式特征是缺少一些在随后的采样时间成为主导的条带。巨大芽孢杆菌和节杆菌。是松散土壤中的优势种群。从根际模式中提取的优势带的测序显示，10条带中有6条与谷物阳性细菌相似。诺卡氏菌种群被鉴定为草莓专一性条带。
The bacterial rhizosphere communities of three host plants of the pathogenic fungus Verticillium dahliae, field-grown strawberry (Fragaria ananassa Duch.), oilseed rape (Brassica napus L.), and potato (Solanum tuberosum L.), were analyzed. We aimed to determine the degree to which the rhizosphere effect is plant dependent and whether this effect would be increased by growing the same crops in two consecutive years. Rhizosphere or soil samples were taken five times over the vegetation periods. To allow a cultivation-independent analysis, total community DNA was extracted from the microbial pellet recovered from root or soil samples. 16S rDNA fragments amplified by PCR from soil or rhizosphere bacterium DNA were analyzed by denaturing gradient gel electrophoresis (DGGE). The DGGE fingerprints showed plant-dependent shifts in the relative abundance of bacterial populations in the rhizosphere which became more pronounced in the second year. DGGE patterns of oilseed rape and potato rhizosphere communities were more similar to each other than to the strawberry patterns. In both years seasonal shifts in the abundance and composition of the bacterial rhizosphere populations were observed. Independent of the plant species, the patterns of the first sampling times for both years were characterized by the absence of some of the bands which became dominant at the following sampling times. Bacillus megaterium and Arthrobacter sp. were found as predominant populations in bulk soils. Sequencing of dominant bands excised from the rhizosphere patterns revealed that 6 out of 10 bands resembled grain-positive bacteria. Nocardia populations were identified as strawberry-specific bands.