Why the dish makes a difference: Quantitative comparison of polystyrene culture surfaces
Why the dish makes a difference: Quantitative comparison of polystyrene culture surfaces
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DOI：
10.1016/j.actbio.2013.02.035
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发表时间：
2013-07-01
影响因子：
9.7
通讯作者：
Van Vliet, Krystyn J.
中科院分区：
文献类型：
--
作者：
Zeiger, Adam S.;Hinton, Benjamin;Van Vliet, Krystyn J.
有广泛的轶事承认，生物细胞的活力和行为可以显著不同的功能来源的商业组织培养聚苯乙烯（TCPS）培养容器，这些细胞粘附。然而，这种明显的材料依赖性通常是通过选择并持续使用同一制造商的产品来解决的-遵循协议-而不是通过调查可能导致这种实验变化的材料特性。在这里，我们通过使用基于原子力显微镜（AFM）的成像和分析、角形测量和蛋白质吸附定量，量化了从广泛的商业来源和加工步骤中获得的TCPS表面的几种物理性质。我们确定了表面特征的定性差异，以及表面粗糙度和润湿性的定量差异，这些差异不能仅仅归因于表面化学的差异。我们还发现在不同TCPS表面培养的细胞在细胞形态和增殖方面存在显著差异，并解决了纳米级表面粗糙度与两种细胞类型细胞增殖率之间的相关性。有趣的是，这些纳米纹理表面上的活贴壁细胞的AFM图像显示了细胞突起和地形特征之间的直接相互作用。这些结果说明并量化了这些普遍存在的材料之间材料表面特性的显着差异，使我们能够更好地理解为什么培养皿可以在生物实验中发挥作用。(C) 2013材料学报Elsevier Ltd.出版。版权所有。
There is wide anecdotal recognition that biological cell viability and behavior can vary significantly as a function of the source of commercial tissue culture polystyrene (TCPS) culture vessels to which those cells adhere. However, this marked material dependency is typically resolved by selecting and then consistently using the same manufacturer's product - following protocol - rather than by investigating the material properties that may be responsible for such experimental variation. Here, we quantified several physical properties of TCPS surfaces obtained from a wide range of commercial sources and processing steps, through the use of atomic force microscopy (AFM)-based imaging and analysis, goniometry and protein adsorption quantification. We identify qualitative differences in surface features, as well as quantitative differences in surface roughness and wettability that cannot be attributed solely to differences in surface chemistry. We also find significant differences in cell morphology and proliferation among cells cultured on different TCPS surfaces, and resolve a correlation between nanoscale surface roughness and cell proliferation rate for both cell types considered. Interestingly, AFM images of living adherent cells on these nanotextured surfaces demonstrate direct interactions between cellular protrusions and topographically distinct features. These results illustrate and quantify the significant differences in material surface properties among these ubiquitous materials, allowing us to better understand why the dish can make a difference in biological experiments. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.