INTERACTION OF PROPIONIBACTERIUM-ACNES WITH SKIN LIPIDS IN-VITRO
INTERACTION OF PROPIONIBACTERIUM-ACNES WITH SKIN LIPIDS IN-VITRO
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DOI：
10.1099/00221287-139-8-1745
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发表时间：
1993-08-01
期刊：
影响因子：
--
通讯作者：
HOLLAND, KT
中科院分区：
文献类型：
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作者：
GRIBBON, EM;CUNLIFFE, WJ;HOLLAND, KT
痤疮丙酸杆菌存在于人体皮肤皮脂富集区毛囊内的优势微生物中。本研究利用连续培养技术研究了已知的皮脂疏水成分对这种微生物的生理和营养的影响，该微生物在3.3摄氏度的条件下厌氧生长。所用的培养基由8种氨基酸组成，以葡萄糖为主要碳源，培养pH维持在5.6。皮脂的测定范围是以C18单不饱和脂肪酸9-顺十八烯酸(油酸)为基础的。在有无葡萄糖存在和不存在的情况下，将原料微粉化的溶液无菌地冲击到连续培养中，并监测营养效果。无论是在最大比生长率(Mu(Max))还是最终培养生物量方面，脂肪底物对痤疮假单胞菌的生长都没有显著影响。与葡萄糖相比，甘油(3mgml-1)是一种较差的碳源/能量来源。然而，细菌细胞确实对不同的脂类有不同程度的黏附，最大的黏附发生在游离脂肪酸上。使用[C-14]油酸的初步摄取实验证实了这一观察结果。在电子显微镜研究中发现，细胞黏附的相互作用部位可能是与痤疮假单胞菌细胞表面相关的脂纤维层。这项研究的结果表明，痤疮假单胞菌脂肪酶的一个功能可能是通过促进细胞对油酸等成分的黏附，帮助毛囊内的定植。
Propionibacterium acnes in the predominant microbial resident within the pilosebaceous follicles of sebum-rich areas of human skin. This study investigated the effects of known hydrophobic components of sebum on the physiology and nutrition of this microorganism, grown anaerobically at 3.3-degrees-C, under defined conditions using continuous culture techniques. The medium used was chemically defined, comprising eight amino acids, with glucose as the main carbon energy source and the culture pH was maintained at 5.6. The range of sebum lipids assayed was based on the C18 monounsaturated fatty acid 9-cis-octadecenoic acid (oleic acid). Stock micronized solutions were aseptically pulsed into continuous cultures in the presence and absence of glucose, and nutritional effects monitored. None of the lipid substrates significantly affected P. acnes growth either in terms of maximum specific growth rate (mu(max)) or final culture biomass yield. Glycerol (3 mg ml-1) was found to be a poor carbon/energy source in comparison to glucose. Bacterial cells did, however, adhere with varying degrees, to the different lipid species, with maximum adherence occurring with the free fatty acid. This observation was confirmed by preliminary uptake experiments using [C-14]oleic acid. The interactive site for cell adherence may be the lipid-fibrillar layer associated with the cell surface of P. acnes, as discerned in electron microscopical studies. The findings of this investigation suggest that one function of the P. acnes lipase may be to aid colonization within the pilosebaceous follicle, by promoting cell adherence to components such as oleic acid.