Interactions of exposure time and temperature on thermostability and protein content of excisedIllicium parviflorum roots
Interactions of exposure time and temperature on thermostability and protein content of excisedIllicium parviflorum roots
复制标题
暴露时间和温度对离体八角根热稳定性和蛋白质含量的相互作用
作者：
D. Ingram;P. F. Webb;R. Biggs
建立了一个模型来描述暴露时间和处理对零星八角热稳定性的交互影响。根细胞膜使用电解质渗漏(LC)程序。将根从25℃移至35℃至60℃之间的处理温度30~300分钟。在选定的暴露时间，LC随温度的升高而增加，而致死暴露时间随温度的升高呈指数下降。利用该技术处理15min的致死温度(52.0±1.1℃)与根暴露于逐渐升高的温度(4℃/h)时的临界温度(52.2±1.2℃)相当。随着温度从35℃升高到40℃，根系总蛋白质含量开始下降，总蛋白质含量下降50%对应的温度为49.1±2.2℃。
SummaryA model was developed to describe interactive effects of exposure time and treatment on thermostability of excisedIllicium parviflorum Michx. root cell membranes using electrolyte leakage (Lc) procedures. Roots were moved from 25°C to treatment temperatures between 35°C and 60°C for 30 to 300 min. A sigmoidal response described Lc increases with increasing temperature at selected time exposures and the lethal exposure time decreased exponentially as temperature increased. The lethal temperature (52.0±1.1°C) for a 15 min exposure using this technique was comparable to the critical temperature (52.2±1.2°C) when roots were exposed to gradually increasing temperatures (4°C per h). Total protein content of roots began to decrease as temperatures increased from 35 to 40°C and the temperature corresponding to 50% reduction in total proteins was 49.1±2.2°C.