Persistence of the 2009 Pandemic Influenza A (H1N1) Virus in Water and on Non-Porous Surface
Persistence of the 2009 Pandemic Influenza A (H1N1) Virus in Water and on Non-Porous Surface
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DOI：
10.1371/journal.pone.0028043
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发表时间：
2011-11-23
期刊：
影响因子：
3.7
通讯作者：
Manuguerra, Jean-Claude
中科院分区：
文献类型：
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作者：
Dublineau, Amelie;Batejat, Christophe;Manuguerra, Jean-Claude
了解甲型流感病毒在不同环境条件下的生存是卫生当局实施卫生和个人保护措施的关键因素。由于它依赖于病毒分离株，即使在同一亚型中，我们研究了2009年H1N1大流行（H1N1pdm）病毒在水和非多孔表面的存活。随着时间的推移，h1n1 - pdm病毒受到各种环境参数的影响，并测试了其传染性。在低、中盐度和4摄氏度的水中，病毒至少存活了200天。温度和盐度的升高对病毒的存活产生了强烈的负面影响，病毒在35摄氏度和盐含量为千分之270 （ppt）的情况下，其传染性不超过1天。基于模型数据，在35℃条件下，甲型h1n1流感病毒在光滑的无孔表面上保持感染性至少7天，在4℃条件下保持感染性长达66天。因此，即使在35℃条件下，甲型h1n1流感病毒也能在水和玻璃表面上持续较长时间。另外的实验表明，与环境直接接触的外部病毒结构主要参与病毒传染性的丧失。
Knowledge of influenza A virus survival in different environmental conditions is a key element for the implementation of hygiene and personal protection measures by health authorities. As it is dependent on virus isolates even within the same subtype, we studied the survival of the 2009 H1N1 pandemic (H1N1pdm) virus in water and on non-porous surface. The H1N1pdm virus was subjected to various environmental parameters over time and tested for infectivity. In water, at low and medium salinity levels and 4 degrees C, virus survived at least 200 days. Increasing temperature and salinity had a strong negative effect on the survival of the virus which remained infectious no more than 1 day at 35 degrees C and 270 parts per thousand (ppt) of salt. Based on modeled data, the H1N1pdm virus retained its infectivity on smooth non-porous surface for at least 7 days at 35 degrees C and up to 66 days at 4 degrees C. The H1N1pdm virus has thus the ability to persist in water and on glass surface for extended periods of time, even at 35 degrees C. Additional experiments suggest that external viral structures in direct contact with the environment are mostly involved in loss of virus infectivity.