Long-term high-titer neutralizing activity induced by octameric synthetic HIV-1 antigen.
Long-term high-titer neutralizing activity induced by octameric synthetic HIV-1 antigen.
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DOI：
10.1126/science.1925584
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发表时间：
1991-10
期刊：
影响因子：
56.9
通讯作者：
C. Y. Wang;D. Looney;M. Li;A. Walfield;J. Ye;B. Hosein;J. Tam;F. Wong-Staal
中科院分区：
文献类型：
--
作者：
C. Y. Wang;D. Looney;M. Li;A. Walfield;J. Ye;B. Hosein;J. Tam;F. Wong-Staal
用代表人免疫缺陷病毒-1IIIB包膜蛋白主要中和决定簇（PND）的八聚体分支肽免疫3.5年后，观察到同源病毒中和活性滴度（大于1：19，683）。加强免疫在豚鼠中引起持久和有效的抗体，超过由常规牛血清白蛋白缀合物产生的反应100倍。肽长度、保守序列的中心呈递和上游序列的包含有助于免疫原性。还产生了异型中和抗体的滴度（大于1：1，000）。八聚PND肽是一种有前途的获得性免疫缺陷综合征（AIDS）疫苗的方法。
A titer for homologous viral neutralization activity (greater than 1:19,683) was observed after a 3.5-year immunization period with an octameric, branching peptide representing the principal neutralizing determinant (PND) of the human immunodeficiency virus-1IIIB envelope protein. Booster immunizations elicited persistent and potent antibodies in guinea pigs, exceeding responses produced by a conventional bovine serum albumin conjugate by 100-fold. Peptide length, central presentation of a conserved sequence, and inclusion of an upstream sequence contributed to immunogenicity. Titers (greater than 1:1,000) of heterotypic neutralizing antibodies also developed. Octameric PND peptides are a promising approach for an acquired immunodeficiency syndrome (AIDS) vaccine.